Siede W, Eckardt F, Brendel M
Mol Gen Genet. 1983;190(3):406-12. doi: 10.1007/BF00331068.
Using the thermoconditional yeast mutant rev2ts that controls an apparently site-specific step of mutagenic DNA repair it was possible to measure the time course of REV2 dependent UV-induced reversion of the ochre allele his5-2 and recovery of survival for UV-treated stationary phase cells: due to the rev2ts coded protein being active at 23 degrees C, survival and mutation frequencies increased with duration of incubation under permissive conditions in growth medium before the temperature was shifted to 36 degrees C (restrictive temperature). This increase was abolished in the presence of the protein synthesis inhibitor, cycloheximide. Furthermore, the REV2 dependent recovery of survival could be blocked or nearly blocked by cycloheximide added at any time during repair. Therefore, REV2 dependent repair can be characterized as a process requiring concomitant protein synthesis. These findings give further support to the concept that in yeast, mutagenesis involves UV inducible components of DNA repair.
利用控制诱变DNA修复明显位点特异性步骤的温度条件型酵母突变体rev2ts，能够测量REV2依赖的赭石型等位基因his5-2的紫外线诱导回复突变的时间进程以及紫外线处理的静止期细胞的存活恢复情况：由于rev2ts编码的蛋白质在23摄氏度时具有活性，在温度转移至36摄氏度（限制温度）之前，在允许条件下于生长培养基中孵育期间，存活率和突变频率随孵育时间增加。在蛋白质合成抑制剂环己酰亚胺存在的情况下，这种增加被消除。此外，在修复过程中的任何时间添加环己酰亚胺，均可阻断或几乎阻断REV2依赖的存活恢复。因此，REV2依赖的修复可被表征为一个需要伴随蛋白质合成的过程。这些发现进一步支持了酵母中诱变涉及DNA修复的紫外线诱导成分这一概念。
