Ehrlich P H, Moustafa Z A, Justice J C, Harfeldt K E, Kelley R L, Ostberg L
Sandoz Research Institute, E. Hanover, NJ 07936.
Hum Antibodies Hybridomas. 1992 Jan;3(1):2-7.
Four hybridoma cell lines stably secreting human monoclonal antibodies directed against hepatitis B surface antigen have been isolated. The monoclonal antibodies have been characterized by determining several allotypes, measuring affinity for HBsAg, binding to two HBsAg subtypes, and kinetics of antibody binding to solid adsorbed antigen. In addition, the relative position of the epitopes have been determined by competition in binding to HBsAg. The results indicate that human anti-HBsAg monoclonal antibodies of quite different properties can be isolated. Pharmacokinetics of one antibody have been measured in two rhesus monkeys. This monoclonal antibody, OST 577, has been compared to hyperimmune gamma globulins in a Bureau of Biologics approved radioimmunoassay. It is expected that these antibodies will be useful in preventing and treating hepatitis B.
已分离出四种稳定分泌针对乙型肝炎表面抗原的人单克隆抗体的杂交瘤细胞系。通过确定几种同种异型、测量对乙肝表面抗原（HBsAg）的亲和力、与两种HBsAg亚型的结合以及抗体与固相吸附抗原结合的动力学来对单克隆抗体进行表征。此外，通过与HBsAg结合的竞争来确定表位的相对位置。结果表明，可以分离出性质截然不同的人抗HBsAg单克隆抗体。已在两只恒河猴中测量了一种抗体的药代动力学。在生物制品局批准的放射免疫分析中，将这种单克隆抗体OST 577与超免疫γ球蛋白进行了比较。预计这些抗体将有助于预防和治疗乙型肝炎。
