Vehmeyer K, Eibl H, Unger C
Department of Internal Medicine, University of Göttingen, Germany.
Exp Hematol. 1992 Jan;20(1):1-5.
The effect of the antitumorally active hexadecylphosphocholine (He-PC) on the colony-stimulating factor (CSF)-dependent growth of human hemopoietic progenitor cells was studied. At low concentrations He-PC stimulated the CSF-dependent progenitor cell colony growth of three patients suffering from chronic myeloid leukemia (CML) and of three of six patients without hematological disorders. The stimulating effect was up to eight times that of the control using granulocyte colony-stimulating factor (G-CSF) and twofold in the case of granulocyte-macrophage colony-stimulating factor (GM-CSF), whereas only slight effects were noted when interleukin 3 (IL-3) or the combination of the CSFs was used as an additive. The stimulatory effects observed are far below the He-PC concentrations that are usually required for the in vitro growth arrest of cancer cells. At higher concentrations He-PC displayed suppressive effects, most pronounced in the case of G-CSF-dependent colony growth. At the concentrations investigated, He-PC failed to show any changes in the composition and distribution of specific colonies. He-PC by itself had no mitogenic activity. This indicates that He-PC acts as a co-stimulator. In the cases of myeloproliferative diseases and in the case of a patient without known hematological disorder, removal of accessory cells did not abrogate the He-PC-enhanced colony growth by CSFs. Thus, the stimulatory effect of low-dose He-PC seems not to be mediated by accessory cells.
研究了具有抗肿瘤活性的十六烷基磷酸胆碱（He-PC）对人造血祖细胞集落刺激因子（CSF）依赖性生长的影响。在低浓度下，He-PC刺激了3例慢性髓性白血病（CML）患者和6例无血液系统疾病患者中的3例的CSF依赖性祖细胞集落生长。刺激作用高达使用粒细胞集落刺激因子（G-CSF）时对照的8倍，使用粒细胞-巨噬细胞集落刺激因子（GM-CSF）时为对照的2倍，而当使用白细胞介素3（IL-3）或CSF组合作为添加剂时仅观察到轻微作用。观察到的刺激作用远低于癌细胞体外生长停滞通常所需的He-PC浓度。在较高浓度下，He-PC表现出抑制作用，在G-CSF依赖性集落生长的情况下最为明显。在所研究的浓度下，He-PC未显示特定集落的组成和分布有任何变化。He-PC本身没有促有丝分裂活性。这表明He-PC作为一种共刺激剂起作用。在骨髓增殖性疾病的情况下以及在一名无已知血液系统疾病的患者中，去除辅助细胞并未消除He-PC增强的CSF集落生长。因此低剂量He-PC的刺激作用似乎不是由辅助细胞介导的。
