Chou Y K, Bourdette D N, Offner H, Whitham R, Wang R Y, Hashim G A, Vandenbark A A
Neuroimmunology Research, V.A. Medical Center, Portland, OR 97201.
J Neuroimmunol. 1992 May;38(1-2):105-13. doi: 10.1016/0165-5728(92)90095-3.
T cell sensitization to two myelin components, myelin basic protein (MBP) and myelin proteolipid protein (PLP), may be important to the pathogenesis of multiple sclerosis (MS). Using the limiting dilution assay, we demonstrated that the blood of MS patients had an increased frequency of MBP-reactive T cells compared with normal subjects and patients with other neurological diseases (OND) and rheumatoid arthritis. There was no difference in T cell frequency to a synthetic peptide, PLP139-151, or Herpes simplex virus. Within cerebrospinal fluid (CSF), 37% of IL-2/IL-4-reactive T cell isolates from MS patients responded either to MBP or PLP139-151 while only 5% of similar isolates from OND patients responded to these myelin antigens. The mean relative frequency of MBP-reactive T cells within CSF from MS patients was significantly higher than that of OND patients (22 x 10(-5) cells versus 1 x 10(-5) cells) and was similar to that of MBP reactive T cells within the central nervous system of rats with experimental autoimmune encephalomyelitis. These results lend new support to the hypothesis that myelin-reactive T cells mediate disease in MS.
T细胞对两种髓鞘成分，即髓鞘碱性蛋白（MBP）和髓鞘蛋白脂蛋白（PLP）的致敏作用，可能在多发性硬化症（MS）的发病机制中起重要作用。通过有限稀释试验，我们证明与正常受试者、其他神经系统疾病（OND）患者及类风湿性关节炎患者相比，MS患者血液中MBP反应性T细胞的频率增加。T细胞对合成肽PLP139 - 151或单纯疱疹病毒的频率没有差异。在脑脊液（CSF）中，MS患者中37%的IL - 2/IL - 4反应性T细胞分离株对MBP或PLP139 - 151有反应，而OND患者中类似分离株只有5%对这些髓鞘抗原有反应。MS患者脑脊液中MBP反应性T细胞的平均相对频率显著高于OND患者（22×10⁻⁵细胞对1×10⁻⁵细胞），且与实验性自身免疫性脑脊髓炎大鼠中枢神经系统内MBP反应性T细胞的频率相似。这些结果为髓鞘反应性T细胞介导MS疾病这一假说提供了新的支持。
