Warren K G, Catz I
Department of Medicine (Neurology), University of Alberta, Edmonton, Canada.
J Neurol Sci. 1994 Jan;121(1):66-73. doi: 10.1016/0022-510x(94)90158-9.
Myelin basic protein (MBP) and proteolipid protein (PLP) were purified from non-MS human brain and used in solid phase radioimmunoassays to detect their specific antibodies in cerebrospinal fluid (CSF) of optic neuritis and clinically definite multiple sclerosis (MS) patients. In 53 optic neuritis patients free anti-MBP was elevated in 47 and in 6 of these 47 patients bound anti-MBP was also increased. The remaining 6 patients with undetectable anti-MBP had increased levels of anti-PLP in their CSF. None of these optic neuritis patients had autoantibodies to both antigens. Of 173 MS patients with acute relapses 169 had increased free anti-MBP. Three of the 4 remaining patients with undetectable anti-MBP had increased anti-PLP in their CSF. Of 110 MS patients with chronic progressive disease, 107 had increased CSF anti-MBP and 2 had elevated anti-PLP. Of 87 MS patients in remission, 15 had modestly elevated anti-MBP and none had detectable anti-PLP. Considering the total of 370 clinically definite MS patients with active and inactive disease, 77% had increased CSF anti-MBP and 1% had increased CSF anti-PLP. These findings are suggesting 2 immunochemically distinct forms of MS: a common form with autoantibodies directed against MBP and a more rare form associated with anti-PLP.
从非多发性硬化症（MS）患者的人脑组织中纯化出髓鞘碱性蛋白（MBP）和蛋白脂蛋白（PLP），并用于固相放射免疫分析，以检测视神经炎和临床确诊的多发性硬化症（MS）患者脑脊液（CSF）中的特异性抗体。在53例视神经炎患者中，47例游离抗MBP升高，其中6例结合抗MBP也升高。其余6例抗MBP检测不到的患者脑脊液中抗PLP水平升高。这些视神经炎患者均无针对两种抗原的自身抗体。在173例急性复发的MS患者中，169例游离抗MBP升高。其余4例抗MBP检测不到的患者中，3例脑脊液中抗PLP升高。在110例慢性进展性疾病的MS患者中，107例脑脊液抗MBP升高，2例抗PLP升高。在87例缓解期的MS患者中，15例抗MBP轻度升高，无抗PLP检测到。考虑到370例临床确诊的MS患者，包括活动期和非活动期，77%的患者脑脊液抗MBP升高，1%的患者脑脊液抗PLP升高。这些发现提示MS存在两种免疫化学上不同的形式：一种常见形式是针对MBP的自身抗体，另一种较罕见的形式与抗PLP有关。
