George J M, Clayton D F
Laboratory of Animal Behavior, Rockefeller University, New York, NY 10021.
Brain Res Mol Brain Res. 1992 Feb;12(4):323-9. doi: 10.1016/0169-328x(92)90134-w.
An RNA identified by differential cDNA cloning (HAT-2) is highly enriched in canary forebrain in areas associated with the control of complex learned behaviors and higher perceptual processes. The nucleotide sequence predicts a protein that is 96% identical to the product of the n-chimaerin gene isolated from human brain and contains two identifiable domains suggesting a novel role in signal transduction processes. One domain is similar to the sequence in protein kinase C which mediates diacylglycerol binding and regulation. The second domain is similar to a portion of BCR, a GTPase-activating protein encoded by the breakpoint cluster region gene. In male canaries examined during the song season, HAT-2 RNA shows variable expression within the song control circuit, and is notably less abundant in the three nuclei which concentrate androgens (HVC, RA and L-MAN). A fundamental function in the vertebrate forebrain and a possible role in the regulation of neural plasticity are suggested by the conserved structure and pattern of expression of this gene in the brain.
通过差异cDNA克隆鉴定出的一种RNA（HAT-2）在金丝雀前脑与复杂学习行为控制及高级感知过程相关的区域高度富集。核苷酸序列预测出一种蛋白质，它与从人脑中分离出的n-嵌合蛋白基因的产物有96%的同一性，并且包含两个可识别的结构域，表明其在信号转导过程中具有新功能。一个结构域类似于蛋白激酶C中介导二酰基甘油结合和调节的序列。第二个结构域类似于BCR的一部分，BCR是由断裂簇区域基因编码的一种GTP酶激活蛋白。在鸣叫季节检查的雄性金丝雀中，HAT-2 RNA在鸣叫控制回路中表现出可变表达，并且在三个浓缩雄激素的核（HVC、RA和L-MAN）中明显较少。该基因在脑中保守的结构和表达模式提示了其在脊椎动物前脑中的基本功能以及在神经可塑性调节中的可能作用。
