Wagner S, Klause U, Mauch H
Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Berlin, Federal Republic of Germany.
Med Microbiol Immunol. 1992;181(1):25-33. doi: 10.1007/BF00193393.
Monoclonal antibodies (mAbs) against Mycobacterium avium were produced which specifically reacted with cell walls of M. avium. The binding pattern was not limited to one subtype. The three most specific mAbs showed binding to the outer surface of M. avium but not to other mycobacterial or bacterial cell surfaces. The combined results of enzyme-linked immunosorbent assay, immunoblot and dot blot showed that mAb 4A006 bound to an epitope located in the cell wall and on the cell surface and mAb 4A010 to an epitope exposed on the cell surface and the cytoplasm. The mAb 4A009-binding epitope was only detectable on the cell surface but not in the cell wall or cytoplasmic fractions of M. avium. In the immunoblot technic a protein antigen with a molecular mass of 27-29 kDa was identified by the mAbs. The mAb 4A006 reacted with 142 out of 143 M. avium subtypes 1, 4 and 8 obtained from AIDS patients. These mAbs seem to be applicable for the identification of M. avium complex after culture.
制备了针对鸟分枝杆菌的单克隆抗体（mAb），这些抗体能与鸟分枝杆菌的细胞壁发生特异性反应。结合模式不限于一种亚型。三种最具特异性的单克隆抗体显示与鸟分枝杆菌的外表面结合，但不与其他分枝杆菌或细菌细胞表面结合。酶联免疫吸附测定、免疫印迹和斑点印迹的综合结果表明，单克隆抗体4A006与位于细胞壁和细胞表面的一个表位结合，单克隆抗体4A010与暴露在细胞表面和细胞质中的一个表位结合。单克隆抗体4A009结合的表位仅在细胞表面可检测到，而在鸟分枝杆菌的细胞壁或细胞质组分中未检测到。在免疫印迹技术中，单克隆抗体鉴定出一种分子量为27 - 29 kDa的蛋白质抗原。单克隆抗体4A006与从艾滋病患者获得的143株鸟分枝杆菌1、4和8亚型中的142株发生反应。这些单克隆抗体似乎适用于培养后鸟分枝杆菌复合群的鉴定。
