Nishimori K, Yugi H, Naiki M, Sugimura T, Tanaka Y, Nonomura I, Yokomizo Y, Kubo S
Infect Immun. 1987 Mar;55(3):711-5. doi: 10.1128/iai.55.3.711-715.1987.
Serovar-specific monoclonal antibodies against Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex serovars 4, 8, and 9 were prepared. Nine, four, and one monoclonal antibodies, respectively, to the serovars were prepared by the usual cell fusion technique. All nine monoclonal antibodies to serovar 4 were monospecific for their homologous serovar and reacted with several native glycopeptidolipids (GPLs) and one major deacylated GPL from the homologous serovar. One of the four monoclonal antibodies to serovar 8 seemed to be monospecific for its homologous serovar, but the other cross-reacted with serovar 6 because serovar 6 organisms contain the same components as does the major deacylated GPL from serovar 8. One monoclonal antibody to serovar 9 was monospecific for its homologous serovar and reacted with one of the two major deacylated GPLs from this serovar. These antibody preparations proved useful for serovar identification.
制备了针对鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌复合群血清型4、8和9的血清型特异性单克隆抗体。分别通过常规细胞融合技术制备了针对这些血清型的9种、4种和1种单克隆抗体。针对血清型4的所有9种单克隆抗体对其同源血清型具有单特异性，并与几种天然糖肽脂（GPL）以及来自同源血清型的一种主要脱酰基GPL发生反应。针对血清型8的4种单克隆抗体中的一种似乎对其同源血清型具有单特异性，但另一种与血清型6发生交叉反应，因为血清型6的菌株含有与血清型8的主要脱酰基GPL相同的成分。针对血清型9的一种单克隆抗体对其同源血清型具有单特异性，并与该血清型的两种主要脱酰基GPL之一发生反应。这些抗体制剂被证明可用于血清型鉴定。
