Gu Z W, Zhong B Z, Nath B, Whong W Z, Wallace W E, Ong T M
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888.
Mutat Res. 1992 May 1;279(1):55-60.
Micronucleus induction and phagocytosis in V79 and CHO cells treated with diesel emission particles (DEP) were studied. After separation of the sample into supernatant and sediment fractions, the genotoxic activity of DEP was shown to reside in the supernatant fraction for the DMSO-extracted sample, and in the sedimented fraction for the dipalmitoyl lecithin (DPL), a primary component of pulmonary surfactant, dispersed sample. More particles from DMSO sediment samples were phagocytized than DPL sediment by both types of cells. This had no effect, however, on micronucleus induction. CHO cells phagocytized fewer particles, but gave a higher number of micronuclei than V79 cells. CHO cells seem to be more sensitive to DEP. Evidently, micronucleus induction is not the result of phagocytosis per se, but is due to the different response of the indicator cells to the DEP sample tested. These results further indicate that most, if not all, genotoxic compounds associated with DEP can be extracted by DMSO and that genotoxic activity associated with DEP inhaled into the lung may also be expressed by dispersion of particles in pulmonary surfactant.
研究了用柴油排放颗粒（DEP）处理的V79和CHO细胞中的微核诱导和吞噬作用。将样品分离成上清液和沉淀部分后，对于用二甲基亚砜（DMSO）提取的样品，DEP的遗传毒性活性存在于上清液部分；而对于肺表面活性剂的主要成分二棕榈酰卵磷脂（DPL）分散的样品，其遗传毒性活性存在于沉淀部分。两种细胞吞噬的DMSO沉淀样品中的颗粒均比DPL沉淀中的颗粒多。然而，这对微核诱导没有影响。CHO细胞吞噬的颗粒较少，但产生的微核数量比V79细胞多。CHO细胞似乎对DEP更敏感。显然，微核诱导不是吞噬作用本身的结果，而是由于指示细胞对所测试的DEP样品的不同反应。这些结果进一步表明，与DEP相关的大多数（如果不是全部）遗传毒性化合物可以被DMSO提取，并且吸入肺部的DEP相关的遗传毒性活性也可能通过颗粒在肺表面活性剂中的分散来表达。
