Keane M J, Xing S G, Harrison J C, Ong T, Wallace W E
National Institute for Occupational Safety and Health, ALOSH, Morgantown, WV 26505.
Mutat Res. 1991 Jul;260(3):233-8. doi: 10.1016/0165-1218(91)90031-g.
Diesel-exhaust particles from two sources were dispersed in aqueous mixtures of dipalmitoyl phosphatidyl choline, a major component of pulmonary surfactant, and were tested for genotoxicity. Diesel samples from the same sources were extracted with dichloromethane and transferred into dimethyl sulfoxide and subjected to the same assays. Both types of extractions yielded similar results in both the Salmonella mutagenicity assay and the sister-chromatid exchange assay using V79 cells. After separation of the samples into supernatant and sediment fractions, the activity of both diesel samples was shown to reside exclusively in the supernatant fraction for the solvent-extracted samples, and exclusively in the sedimented fraction for surfactant dispersed samples. These findings indicate that genotoxic activity associated with diesel particles inhaled into the lung may be made bioavailable by virtue of the solubilization/dispersion properties of pulmonary surfactant components.
来自两个来源的柴油废气颗粒被分散在二棕榈酰磷脂酰胆碱（肺表面活性剂的主要成分）的水性混合物中，并进行了遗传毒性测试。从相同来源采集的柴油样品用二氯甲烷萃取，转移到二甲基亚砜中，并进行相同的检测。在沙门氏菌诱变试验和使用V79细胞的姐妹染色单体交换试验中，两种萃取类型都产生了相似的结果。将样品分离成上清液和沉淀部分后，溶剂萃取样品的两种柴油样品的活性仅存在于上清液部分，而表面活性剂分散样品的活性仅存在于沉淀部分。这些发现表明，吸入肺部的柴油颗粒相关的遗传毒性活性可能由于肺表面活性剂成分的溶解/分散特性而具有生物可利用性。
