SALISBURY G W, BIRGE W J, DE LA TORRE L, LODGE J R
J Biophys Biochem Cytol. 1961 Jul;10(3):353-9. doi: 10.1083/jcb.10.3.353.
The Feulgen-DNA content of sperm cells from 5 bulls was studied by means of microspectrophotometry after storage at 5 degrees C for 2, 3, 5, and 10 days in a yolk-citrate diluent permitting slow aerobic metabolism. A subsample of sperm cells from each bull was subjected to the Feulgen technique on each of the storage days selected. The cells sampled on each of these days received a standard 12 minute, 60 degrees C hydrolysis. Absorption measurements at 546 mmicroof the individual cells indicated a marked progressive decrease in the Feulgen-DNA content of the stored spermatozoa. The loss of 30 per cent of the initial DNA at the end of 5 days' storage was highly significant statistically. This decrease approximately parallels the known decrease in fertility of stored sperm cells, as well as the increase in apparent embryonic mortality resulting from the use of similarly aged spermatozoa for artificial insemination.
对5头公牛的精子细胞进行了研究，这些精子细胞保存在允许缓慢有氧代谢的蛋黄柠檬酸盐稀释液中，于5℃下储存2、3、5和10天，之后采用显微分光光度法测定其孚尔根DNA含量。在选定的每个储存日，从每头公牛的精子细胞中抽取一个子样本进行孚尔根技术处理。在这些日子中每天抽取的细胞都接受标准的12分钟、60℃水解处理。对单个细胞在546微米处的吸收测量表明，储存精子的孚尔根DNA含量显著逐渐下降。储存5天后初始DNA损失30%，在统计学上具有高度显著性。这种下降大致与已知的储存精子细胞生育力下降情况以及使用同样老化的精子进行人工授精导致的明显胚胎死亡率增加情况相符。
