聚合酶链反应（PCR）在兽医诊断病毒学中的应用。
Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology.
作者信息
Belák S, Ballagi-Pordány A
机构信息
Department of Virology, National Veterinary Institute, Uppsala, Sweden.
出版信息
Vet Res Commun. 1993;17(1):55-72. doi: 10.1007/BF01839180.
Abstract
The polymerase chain reaction has become an important diagnostic tool for the veterinary virologist. Conventional methods for detecting viral diseases can be laborious or ineffective. In many cases PCR can provide a rapid and accurate test. In this article we explain the basic principles of PCR and supply a reference list of its uses in diagnostic veterinary virology.
摘要
聚合酶链反应已成为兽医病毒学家的一项重要诊断工具。检测病毒性疾病的传统方法可能既费力又无效。在许多情况下，聚合酶链反应可以提供快速准确的检测。在本文中，我们解释了聚合酶链反应的基本原理，并提供了其在兽医诊断病毒学中应用的参考列表。
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本文引用的文献
1
Nucleic acid hybridization-an alternative tool in diagnostic microbiology.
Immunol Today. 1985 Sep;6(9):268-72. doi: 10.1016/0167-5699(85)90065-9.
2
Polymerase chain reaction amplification of segments of pestivirus genomes.
Arch Virol Suppl. 1991;3:231-8. doi: 10.1007/978-3-7091-9153-8_28.
3
Detection of persistent bovine viral diarrhea virus infections by DNA hybridization and polymerase chain reaction assay.通过DNA杂交和聚合酶链反应检测持续性牛病毒性腹泻病毒感染
Arch Virol Suppl. 1991;3:199-208. doi: 10.1007/978-3-7091-9153-8_24.
4
Bovine viral diarrhea virus infection: rapid diagnosis by the polymerase chain reaction.牛病毒性腹泻病毒感染：通过聚合酶链反应进行快速诊断
Arch Virol Suppl. 1991;3:181-90. doi: 10.1007/978-3-7091-9153-8_22.
5
Experiences on the application of the polymerase chain reaction in a diagnostic laboratory.聚合酶链反应在诊断实验室中的应用经验。
Mol Cell Probes. 1993 Jun;7(3):241-8. doi: 10.1006/mcpr.1993.1035.
6
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.通过聚合酶催化的链式反应在体外特异性合成DNA。
Methods Enzymol. 1987;155:335-50. doi: 10.1016/0076-6879(87)55023-6.
7
Molecular diagnosis of infectious diseases by nucleic acid hybridization.通过核酸杂交进行传染病的分子诊断。
Mol Cell Probes. 1987 Mar;1(1):3-14. doi: 10.1016/0890-8508(87)90003-x.
8
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
9
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Vet Rec. 1988 Nov 26;123(22):570-1. doi: 10.1136/vr.123.22.570.
10
Bovine leucosis virus contamination of a vaccine produced in vivo against bovine babesiosis and anaplasmosis.
Aust Vet J. 1988 Sep;65(9):285-7. doi: 10.1111/j.1751-0813.1988.tb16144.x.
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