Becker Y
Department of Molecular Virology, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
Virus Genes. 1992 Apr;6(2):131-41. doi: 10.1007/BF01703062.
The envelope glycoprotein B (gB) coded for by the herpes simplex virus type 1 (HSV-1) UL27 gene is similar to the amino acid (aa) sequence of the gB coded by a homologous gene in HSV-2 DNA. The putative antigenic domains in HSV-1 and HSV-2 gB glycoproteins were analyzed on a comparative basis by suitable computer programs, which allowed the prediction of putative antigenic and topogenic domains. The computer-derived domains were compared to experimentally reported antigenic domains in HSV-1 gB glycoprotein. The computer-predicted antigenic domains in the HSV-1 gB glycoprotein matched well with the reported experimentally derived antigenic domains. The aa sequence of antigenic domain 1 was noted to resemble the amino acid sequence in ApoE that is involved in the attachment of this protein to LDL receptors. The clusters of hydrophobic aa domains are conserved in the two viral glycoproteins and are signals for transfer of the viral proteins through the cellular membrane.
由单纯疱疹病毒1型（HSV-1）UL27基因编码的包膜糖蛋白B（gB）与HSV-2 DNA中同源基因编码的gB氨基酸（aa）序列相似。通过合适的计算机程序对HSV-1和HSV-2 gB糖蛋白中的推定抗原结构域进行了比较分析，从而能够预测推定的抗原结构域和拓扑结构域。将计算机推导的结构域与HSV-1 gB糖蛋白中实验报道的抗原结构域进行比较。HSV-1 gB糖蛋白中计算机预测的抗原结构域与报道的实验推导的抗原结构域匹配良好。注意到抗原结构域1的氨基酸序列类似于载脂蛋白E中参与该蛋白与低密度脂蛋白受体结合的氨基酸序列。疏水氨基酸结构域簇在两种病毒糖蛋白中是保守的，并且是病毒蛋白通过细胞膜转运的信号。
