Rieckhoff-Cantoni L, Bernard P, Didierjean L, Imhof K, Kinloch-de Loës S, Saurat J H
Department of Dermatology, University Hospital, Geneva, Switzerland.
Arch Dermatol. 1992 Jun;128(6):791-4.
Ninety-seven patients suffering from a pruritic dermatosis were screened for the detection of bullous pemphigoid (BP) antibodies (ab) using the Western immunoblot (WB) analysis technique.
Twenty-four patients (25%) reacted at least twice with the BP antigen on WB analysis at a 1/10 dilution: seven had typical BP, four had papular BP, 10 had pruritus and prurigo of unknown origin, two had eczema, and one had lichen planus. This corresponds to 13% of BP-type ab in patients who did not fulfill criteria for BP. Therefore, we did the following: (1) tested a control group of 24 subjects; (2) assessed the reproducibility of the WB method by retesting the same serum samples on different epidermal extracts; and (3) estimated the BP ab titer. None of the 24 control subjects had detectable BP ab, and reproducible results were obtained in all groups when serum samples were retested at the 1/10 dilution. Although 86% (6/7) of patients with BP had BP ab titers of 1/100 or greater, only 60% (6/10) of the group with pruritus and prurigo and 33% (1/3) of the group with eczema reached such titers.
These results indicate that due to its sensitivity, the WB method can detect low titers of BP ab in patients with pruritic dermatoses who did not fulfill criteria for BP, and therefore we question the specificity of this method.
采用蛋白质免疫印迹（WB）分析技术，对97例患有瘙痒性皮肤病的患者进行大疱性类天疱疮（BP）抗体检测。
24例患者（25%）在WB分析中，以1/10稀释度时与BP抗原至少发生两次反应：7例患有典型BP，4例患有丘疹性BP，10例患有不明原因的瘙痒症和痒疹，2例患有湿疹，1例患有扁平苔藓。这相当于不符合BP标准的患者中BP型抗体的13%。因此，我们进行了以下操作：（1）检测24名受试者的对照组；（2）通过在不同的表皮提取物上重新检测相同的血清样本，评估WB方法的可重复性；（3）估计BP抗体滴度。24名对照受试者均未检测到可检测的BP抗体，当血清样本以1/10稀释度重新检测时，所有组均获得了可重复的结果。虽然86%（6/7）的BP患者BP抗体滴度为1/100或更高，但只有60%（6/10）的瘙痒症和痒疹组以及33%（1/3）的湿疹组达到了这样的滴度。
这些结果表明，由于其敏感性，WB方法可以在不符合BP标准的瘙痒性皮肤病患者中检测到低滴度的BP抗体，因此我们对该方法的特异性提出质疑。
