Ishikawa M, Hayashi M
Department of Biology, Ochanomizu University, Tokyo, Japan.
Biochim Biophys Acta. 1992 May 22;1121(1-2):173-7. doi: 10.1016/0167-4838(92)90351-d.
The present study describes that the collagen-binding activity of vitronectin in human serum increases by treatment with heparin, heating and urea. Vitronectin purified from human serum was bound to native collagen, whereas endogenous vitronectin in the serum was not. We have examined the conditions to change the collagen-binding activity of endogenous vitronectin. Endogenous vitronectin in human serum became considerably bound to collagen when the serum was boiled in 4-8 M urea for 5 min and mixed with heparin (0.5-5 micrograms/ml). Each treatment of heating, urea or heparin alone, and any combination of the two factors, inefficiently activated the binding. Dextran sulfate could substitute for heparin, but dermatan sulfate, keratan sulfate, chondroitin sulfate A and C, heparan sulfate and hyaluronan could not. Possible explanations for the activation of endogenous vitronectin are discussed.
本研究描述了人血清中玻连蛋白的胶原结合活性通过肝素处理、加热和尿素处理而增加。从人血清中纯化的玻连蛋白与天然胶原结合，而血清中的内源性玻连蛋白则不结合。我们已经研究了改变内源性玻连蛋白胶原结合活性的条件。当人血清在4-8M尿素中煮沸5分钟并与肝素（0.5-5微克/毫升）混合时，血清中的内源性玻连蛋白与胶原大量结合。单独的加热、尿素或肝素处理，以及这两种因素的任何组合，都不能有效地激活结合。硫酸葡聚糖可以替代肝素，但硫酸皮肤素、硫酸角质素、硫酸软骨素A和C、硫酸乙酰肝素和透明质酸则不能。对内源性玻连蛋白激活的可能解释进行了讨论。
