Hardwick C, Hoare K, Owens R, Hohn H P, Hook M, Moore D, Cripps V, Austen L, Nance D M, Turley E A
Department of Biochemistry, University of Alabama, Birmingham 35233.
J Cell Biol. 1992 Jun;117(6):1343-50. doi: 10.1083/jcb.117.6.1343.
A cDNA encoding a unique hyaluronan receptor has been molecularly cloned from a lambda GT11 3T3 cDNA expression library. Immunoblot analyses of cell lysates, using antibodies to peptides encoded in the cDNA, specifically react with a 58-kD protein. This protein is regulated by the mutant H-ras gene in cells containing a metallothionein promoter H-ras hybrid gene. Further, antibodies to peptide sequences encoded in the cDNA block the increase in locomotion resulting from induction of the mutant H-ras gene in this cell line. In a transblot assay, the bacterially expressed protein binds to biotinylated hyaluronan. Antibodies to peptides encoded in the cDNA react in immunoblot assays with the 58- and 52-kD proteins of a novel hyaluronan receptor complex previously implicated in cell locomotion. Furthermore, antibodies specific to the 58- and 52-kD proteins, which block ras-induced locomotion, also cross-react with the expressed, encoded protein. The gene product described here appears to be a new type of hyaluronan receptor that is involved in cell locomotion. It is named RHAMM, an acronym for receptor for hyaluronan-mediated motility.
已从λGT11 3T3 cDNA表达文库中分子克隆出一种编码独特透明质酸受体的cDNA。使用针对该cDNA编码肽段的抗体对细胞裂解物进行免疫印迹分析，结果显示该抗体与一种58 kDa的蛋白质发生特异性反应。在含有金属硫蛋白启动子H-ras杂交基因的细胞中，这种蛋白质受突变型H-ras基因调控。此外，针对该cDNA编码肽段序列的抗体可阻断此细胞系中因突变型H-ras基因诱导而导致的运动增加。在转印分析中，细菌表达的蛋白质可与生物素化的透明质酸结合。针对该cDNA编码肽段的抗体在免疫印迹分析中与一种先前认为与细胞运动有关的新型透明质酸受体复合物的58 kDa和52 kDa蛋白质发生反应。此外，可阻断ras诱导运动的针对58 kDa和52 kDa蛋白质的特异性抗体也与表达的编码蛋白发生交叉反应。此处描述的基因产物似乎是一种参与细胞运动的新型透明质酸受体。它被命名为RHAMM，即透明质酸介导运动受体（receptor for hyaluronan-mediated motility）的首字母缩写。
