一种从微生物中大规模制备线粒体的简单方法。 - Suppr

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A simple method for the large-scale preparation of mitochondria from microorganisms.

作者信息

Lang B, Burger G, Doxiadis I, Thomas D Y, Bandlow W, Kaudewitz F

出版信息

Anal Biochem. 1977 Jan;77(1):110-21. doi: 10.1016/0003-2697(77)90295-0.

DOI:10.1016/0003-2697(77)90295-0

PMID:137681

Abstract

摘要

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A simple method for the large-scale preparation of mitochondria from microorganisms.一种从微生物中大规模制备线粒体的简单方法。

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Large-scale preparation of yeast mitochondria exhibiting acceptor control.大规模制备具有呼吸控制作用的酵母线粒体。

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Transformation of Saccharomyces cerevisiae with plasmids containing fragments of yeast 2 μ DNA and a suppressor tRNA gene.用含有酵母 2 μ DNA 片段和一个抑制 tRNA 基因的质粒转化酿酒酵母。

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Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage.与酵母线粒体以及噬菌体T3和T7 RNA聚合酶同源的序列广泛存在于整个真核生物谱系中。

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