Marcovistz R, Decote Ricardo D, Gonçalves da Costa S C
Departamento de Imunologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brasil.
Pathol Biol (Paris). 1992 Mar;40(3):234-7.
This study describes the production and action of interferon in mice infected with Colombian and Y strain T. cruzi. The production of interferon was monitored by an in vitro assay of plasma and extract of spleen, lung and heart for interferon activity. The action of interferon in mice was assessed by measuring an interferon-mediated enzyme activity, 2-5A synthetase. Infected mice (strain Balb/c) were sacrificed at different time intervals, and the level of this enzyme was measured in extracts of spleen, lung and heart. Colombian strain infection induced higher levels of interferon than Y strain under the same conditions; consequently, a greater increase in 2-5A synthetase induction was observed in the former of the two strains. These results suggest that interferon produced by T. cruzi infected mice is active, since a variety of organs respond to its presence by producing elevated levels of 2-5A synthetase.
本研究描述了感染哥伦比亚株和Y株克氏锥虫的小鼠体内干扰素的产生及作用。通过对血浆以及脾脏、肺和心脏提取物进行体外干扰素活性测定来监测干扰素的产生。通过测量干扰素介导的酶活性（2-5A合成酶）来评估干扰素在小鼠体内的作用。在不同时间间隔处死感染小鼠（Balb/c品系），并测量脾脏、肺和心脏提取物中该酶的水平。在相同条件下，哥伦比亚株感染诱导产生的干扰素水平高于Y株；因此，在这两种菌株中，前者观察到2-5A合成酶诱导的增加幅度更大。这些结果表明，克氏锥虫感染小鼠产生的干扰素具有活性，因为多种器官会因干扰素的存在而产生升高水平的2-5A合成酶来做出反应。
