Galabru J, Robert N, Buffet-Janvresse C, Rivière Y, Hovanessian A G
J Gen Virol. 1985 Apr;66 ( Pt 4):711-8. doi: 10.1099/0022-1317-66-4-711.
Two interferon-mediated enzymes, a 2-5A synthetase and a kinase that phosphorylates a 67 000 mol. wt. (p67K) protein were found at variable levels in different organs of mice. Among the different strains of mice included in this study, germ-free mice had the lowest levels of these enzymes. The levels of 2-5A synthetase and p67K kinase were enhanced significantly in all mice following treatment with mouse (alpha + beta) interferon. Here, we show that the presence of 2-5A synthetase and p67K kinase in different organs of normal mice (untreated) was due, at least in part, to a constant production of interferon under different physiological conditions. Accordingly, injection of normal mice with anti-mouse interferon (alpha + beta) globulin led to a significant decrease in the level of 2-5A synthetase and p67K kinase. In conventional mice (C3H/He), the level of both of these enzymes was higher in female than in male animals and was decreased with age or when such animals were reared isolated in a pathogen-free protected unit. The levels of 2-5A synthetase and p67K kinase were also decreased in normal mice following injection with a powerful antibiotic against a very wide spectrum of Gram-positive and Gram-negative bacteria. These results suggest that the production of interferon was induced continuously in normal mice. Such induction was mediated by both internal and external agents.
在小鼠的不同器官中发现了两种干扰素介导的酶，一种2-5A合成酶和一种能磷酸化67000分子量（p67K）蛋白质的激酶，其水平各不相同。在本研究纳入的不同品系小鼠中，无菌小鼠的这些酶水平最低。在用小鼠（α+β）干扰素处理后，所有小鼠体内的2-5A合成酶和p67K激酶水平均显著提高。在此，我们表明正常小鼠（未处理）不同器官中2-5A合成酶和p67K激酶的存在，至少部分归因于在不同生理条件下持续产生的干扰素。因此，给正常小鼠注射抗小鼠干扰素（α+β）球蛋白会导致2-5A合成酶和p67K激酶水平显著降低。在常规小鼠（C3H/He）中，这两种酶在雌性动物中的水平高于雄性动物，并且随着年龄增长或当这些动物在无病原体的保护单元中单独饲养时会降低。在用针对多种革兰氏阳性和革兰氏阴性细菌的强效抗生素注射后，正常小鼠体内的2-5A合成酶和p67K激酶水平也会降低。这些结果表明正常小鼠体内持续诱导产生干扰素。这种诱导由内部和外部因素介导。
