一项关于甘油冷冻红细胞在-80摄氏度下储存长达37年的实验。
An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years.
作者信息
Valeri C R, Ragno G, Pivacek L E, Cassidy G P, Srey R, Hansson-Wicher M, Leavy M E
机构信息
Naval Blood Research Laboratory, Boston University School of Medicine, Boston, MA 02118, USA.
出版信息
Vox Sang. 2000;79(3):168-74. doi: 10.1159/000031236.
BACKGROUND AND OBJECTIVES
Red cells frozen using 40% W/V glycerol are currently FDA approved for frozen storage at -80 degrees C for up to 10 years.
MATERIALS AND METHODS
Red cells frozen with 40% W/V glycerol and stored at -80 degrees C for up to 37 years were thawed, deglycerolized, and stored at 4 degrees C for 24 h.
RESULTS
Red cells frozen for up to 37 years had mean freeze-thaw-wash recovery values of 75%, less than 1% hemolysis, and normal ATP, 2,3-DPG and P50 levels, and 60% of normal RBC K(+) levels.
CONCLUSIONS
Red cells frozen with 40% W/V glycerol can be stored at -80 degrees C for up to 37 years with acceptable in vitro results.
背景与目的
目前，美国食品药品监督管理局（FDA）批准使用40%（重量/体积）甘油冷冻的红细胞可在-80℃下冷冻保存长达10年。
材料与方法
将用40%（重量/体积）甘油冷冻并在-80℃下保存长达37年的红细胞解冻、去甘油，然后在4℃下保存24小时。
结果
冷冻长达37年的红细胞平均冻融洗涤回收率为75%，溶血率低于1% , 三磷酸腺苷（ATP）、2,3-二磷酸甘油酸（2,3-DPG）和P50水平正常，红细胞钾离子（RBC K(+)）水平为正常水平的60%。
结论
用40%（重量/体积）甘油冷冻的红细胞可在-80℃下保存长达37年，体外结果可接受。
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