Prallet B, Male P, Neff L, Baron R
Department of Orthopedics, Yale University School of Medicine, New Haven, Connecticut.
J Bone Miner Res. 1992 Apr;7(4):405-14. doi: 10.1002/jbmr.5650070408.
Mononuclear cells were isolated from the peritrabecular bone marrow from the medullary bone of laying hens maintained on a calcium-deficient diet for 1 week. These cells were cultured for up to 7 days on devitalized bovine bone slices after removing the nonadherent fraction. The mononuclear precursors of the osteoclast that are present in such cultures adhere to bone matrix. These cells are TRAP+, express the vitronectin receptor at high levels, and also express high levels of sodium pumps and of carbonic anhydrase, enzymes that are characteristically enriched in the mature osteoclast. Finally, the most mature mononuclear precursors were found to be capable of resorbing the extracellular bone matrix before forming multinucleated osteoclasts.
从饲喂缺钙日粮1周的产蛋母鸡骨髓骨小梁周围骨髓中分离出单核细胞。去除非贴壁部分后，将这些细胞在失活的牛骨切片上培养长达7天。此类培养物中存在的破骨细胞单核前体细胞粘附于骨基质。这些细胞TRAP阳性，高水平表达玻连蛋白受体，还高水平表达钠泵和碳酸酐酶，这些酶在成熟破骨细胞中典型地富集。最后，发现最成熟的单核前体细胞在形成多核破骨细胞之前能够吸收细胞外骨基质。
