Komisar J, Rivera J, Vega A, Tseng J
Department of Experimental Pathology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
Infect Immun. 1992 Jul;60(7):2969-75. doi: 10.1128/iai.60.7.2969-2975.1992.
Staphylococcal enterotoxin B (SEB) was tested in rodent mast cell cultures for the release of serotonin. Both rat RBL-2H3 mast cells and murine peritoneal cells released serotonin after SEB stimulation in culture. Release of serotonin in RBL-2H3 cells depended on the concentration of SEB; an appreciable release was seen at 50 micrograms/ml. The release of serotonin was not due to cell death. Serotonin release could be enhanced by bradykinin but not by vasoactive intestinal peptide, substance P, lipopolysaccharide from Salmonella typhimurium, the calcium ionophore A23187, acetylcholine, adenosine, 5-hydroxyeicosatetraenoic acid, indomethacin, or phorbol myristate acetate. SEB bound directly to the membrane of RBL-2H3 mast cells, and the SEB-binding site, the presumptive receptor, appeared to be a protein. The SEB receptor could not be capped under membrane-capping conditions, and serotonin release could not be enhanced by attempts to cross-link the receptor. These results suggest that mast cells may be an important cell type involved in SEB toxicosis and that release of serotonin may be enhanced by activation of the kinin-kallikrein system.
在啮齿动物肥大细胞培养物中检测了葡萄球菌肠毒素B（SEB）对血清素释放的影响。在培养过程中，大鼠RBL-2H3肥大细胞和小鼠腹膜细胞在SEB刺激后均释放了血清素。RBL-2H3细胞中血清素的释放取决于SEB的浓度；在50微克/毫升时可观察到明显的释放。血清素的释放并非由于细胞死亡。缓激肽可增强血清素的释放，但血管活性肠肽、P物质、鼠伤寒沙门氏菌的脂多糖、钙离子载体A23187、乙酰胆碱、腺苷、5-羟基二十碳四烯酸、吲哚美辛或佛波醇肉豆蔻酸酯则不能。SEB直接与RBL-2H3肥大细胞膜结合，SEB结合位点（推测为受体）似乎是一种蛋白质。在膜帽形成条件下，SEB受体不能被封帽，并且试图交联受体也不能增强血清素的释放。这些结果表明，肥大细胞可能是参与SEB中毒的一种重要细胞类型，并且激肽-激肽释放酶系统的激活可能会增强血清素的释放。
