Tseng J, Komisar J L, Chen J Y, Hunt R E, Johnson A J, Pitt L, Rivera J, Ruble D L, Trout R, Vega A
Department of Experimental Pathology, Walter Reed Army Institute of Research, Washington, DC 20307.
Infect Immun. 1993 Feb;61(2):391-8. doi: 10.1128/iai.61.2.391-398.1993.
Rhesus monkeys immunized intramuscularly or orally with staphylococcal enterotoxin B (SEB) toxoid or SEB toxoid incorporated in microspheres made of poly(DL-lactide-co-glycolide) were challenged with a lethal dose of aerosolized SEB to study their immunity and cellular responses in the circulation. It was found that circulating antibodies play a critical role in preventing SEB from triggering toxicosis. Monkeys with high levels of antibodies survived, while those with low levels underwent 2 to 3 days of toxicosis and died. Intramuscular immunization induced high levels and oral immunization induced low levels of antibodies. The circulating antibodies in surviving monkeys decreased dramatically within 20 min and started to rebound at 90 min after SEB challenge. At 90 min, the dying monkeys showed in the circulation a dramatic increase of polymorphonuclear leukocytes and decreases of NK cells and monocytes (CD16 and CD56 markers) as well as of lymphocytes with HLA-DR, CD2, CD8, and IL2R alpha (CD25) markers. The number of polymorphonuclear leukocytes showed an inverse correlation with the numbers of monocytes and various lymphocyte subpopulations which, except for IL-2R, CD16, and CD56(+) cells, showed a direct correlation with one another. The changes in the populations of leukocytes, monocytes, NK cells, and lymphocytes seem to be an indication of initial toxicosis; however, the roles of these cells in toxicosis and death remain to be defined.
用葡萄球菌肠毒素B（SEB）类毒素或掺入聚（DL-丙交酯-共-乙交酯）制成的微球中的SEB类毒素对恒河猴进行肌肉注射或口服免疫，然后用致死剂量的雾化SEB进行攻击，以研究它们在循环系统中的免疫和细胞反应。结果发现，循环抗体在预防SEB引发中毒方面起着关键作用。抗体水平高的猴子存活下来，而抗体水平低的猴子则经历2至3天的中毒后死亡。肌肉注射免疫诱导产生高水平抗体，口服免疫诱导产生低水平抗体。存活猴子体内的循环抗体在SEB攻击后20分钟内急剧下降，并在90分钟时开始反弹。在90分钟时，濒死的猴子循环系统中多形核白细胞显著增加，自然杀伤细胞、单核细胞（CD16和CD56标记）以及带有HLA-DR、CD2、CD8和IL2Rα（CD25）标记的淋巴细胞减少。多形核白细胞的数量与单核细胞及各种淋巴细胞亚群的数量呈负相关，除IL-2R、CD16和CD56(+)细胞外，各种淋巴细胞亚群之间呈正相关。白细胞、单核细胞、自然杀伤细胞和淋巴细胞群体的变化似乎表明最初发生了中毒；然而，这些细胞在中毒和死亡中的作用仍有待确定。
