对L-谷氨酰胺和L-天冬酰胺对粗糙脉孢菌NADP特异性谷氨酸脱氢酶影响的重新研究。
Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase.
作者信息
Wootton J C, Kavanagh J P, Baron A J, Lovett M G
出版信息
Biochem J. 1976 Dec 1;159(3):803-6. doi: 10.1042/bj1590803.
Abstract
L-Glutamine, when purified free of traces of NH4+ present in solution, does not act as an alternative substrate to NH4+ for the NADP-specific glutamate dehydrogenase of Neurospora. L-Glutamine interferes with detection of small quantities of NH4+ by Nessler's reagent. L-Asparagine is not an alternative substrate to NH4+ for this enzyme.
摘要
当溶液中纯化的L-谷氨酰胺不含痕量NH4+时，它不会作为NH4+的替代底物，供链孢霉的NADP特异性谷氨酸脱氢酶使用。L-谷氨酰胺会干扰用奈斯勒试剂检测少量NH4+。L-天冬酰胺也不是该酶的NH4+替代底物。
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