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氮源调节粗糙脉孢菌中谷氨酸脱氢酶NADP的合成。

Nitrogen source regulates glutamate dehydrogenase NADP synthesis in Neurospora crassa.

作者信息

Hernández G, Sánchez-Pescador R, Palacios R, Mora J

出版信息

J Bacteriol. 1983 Apr;154(1):524-8. doi: 10.1128/jb.154.1.524-528.1983.

DOI:10.1128/jb.154.1.524-528.1983
PMID:6300039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217491/
Abstract

Neurospora crassa glutamate dehydrogenase-NADP (EC 1.3.1.3) has a higher activity when mycelium is grown on ammonium or nitrate as nitrogen source than when grown on glutamate or glutamine. Quantitative immunoelectrophoresis established that, under all conditions, enzyme activity corresponded to enzyme concentration. Isotope incorporation studies demonstrated that the nitrogen source exerts its regulation at the level of de novo enzyme synthesis.

摘要

粗糙脉孢菌谷氨酸脱氢酶-NADP（EC 1.3.1.3）在以铵或硝酸盐作为氮源生长的菌丝体中，比在以谷氨酸或谷氨酰胺生长时具有更高的活性。定量免疫电泳表明，在所有条件下，酶活性与酶浓度相对应。同位素掺入研究表明，氮源在从头合成酶的水平上发挥其调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/bc60e028ba68/jbacter00245-0537-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/febe1c4aa4fe/jbacter00245-0536-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/0bce973ab385/jbacter00245-0536-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/bc60e028ba68/jbacter00245-0537-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/febe1c4aa4fe/jbacter00245-0536-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/0bce973ab385/jbacter00245-0536-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dae/217491/bc60e028ba68/jbacter00245-0537-a.jpg

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1
Nitrogen source regulates glutamate dehydrogenase NADP synthesis in Neurospora crassa.氮源调节粗糙脉孢菌中谷氨酸脱氢酶NADP的合成。
J Bacteriol. 1983 Apr;154(1):524-8. doi: 10.1128/jb.154.1.524-528.1983.
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本文引用的文献

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Concurrent regulation of glutamic acid dehydrogenases of Neurospora.粗糙脉孢菌谷氨酸脱氢酶的协同调控
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