Bagavandoss P, Mitchell M A, Wilks J W
Cancer and Infectious Diseases Research, Upjohn Laboratories, Kalamazoo, Michigan 49001.
Biotech Histochem. 1992 May;67(3):131-5. doi: 10.3109/10520299209110022.
The binding of histamine to cultured microvascular endothelial cells and glycol methacrylate embedded ovarian tissue sections has been localized using fluorescein-albumin-histamine conjugate. Histamine conjugate was bound to the plasma membranes and nuclei of luteal, endothelial, and ovarian stromal cells. An apparent increase in the binding of histamine to nuclei was observed in the presence of cimetidine but the plasma membrane staining was still evident. Unlike cimetidine, pyrilamine completely inhibited the binding of histamine to the plasma membrane. Instead, in the presence of pyrilamine, histamine bound exclusively to the nuclei of endothelial, germinal epithelial, granulosa, and stromal cells. However, the nuclei of terminally differentiated luteal cells and oocytes were not labeled. The functional significance of these nuclear histamine binding sites remains to be determined.
利用荧光素 - 白蛋白 - 组胺结合物，已确定组胺与培养的微血管内皮细胞以及甲基丙烯酸乙二醇酯包埋的卵巢组织切片的结合部位。组胺结合物与黄体细胞、内皮细胞和卵巢基质细胞的质膜及细胞核相结合。在西咪替丁存在的情况下，观察到组胺与细胞核的结合明显增加，但质膜染色仍然明显。与西咪替丁不同，吡苄明完全抑制组胺与质膜的结合。相反，在吡苄明存在的情况下，组胺仅与内皮细胞、生发上皮细胞、颗粒细胞和基质细胞的细胞核相结合。然而，终末分化的黄体细胞和卵母细胞的细胞核未被标记。这些细胞核组胺结合位点的功能意义尚待确定。
