Hubert J, Seve A P, Bouvier D, Masson C, Bouteille M, Monsigny M
Biol Cell. 1985;55(1-2):15-20. doi: 10.1111/j.1768-322x.1985.tb00404.x.
Mannose-binding sites were detected at the ultrastructural level in nuclei of lizard granulosa cells in situ by means of mannosylated ferritin. When ultrathin sections of material embedded in glycol methacrylate were incubated with mannosylated ferritin, a strong labelling was observed over nucleoli, chromatin and the external leaflet of the nuclear envelope, but no labelling was detected in the perinuclear space except for nuclear pores. This labelling was clearly inhibited when sections were incubated in a solution containing both mannosylated ferritin and a sugar-related neoglycoprotein (mannosylated serum albumin). These ultrastructural data supporting the existence of nuclear lectin-like components in reptilian cells are in agreement with our previous findings about such components in nuclei isolated from mammalian cells. Owing to the unique organization of the granular component of the nucleoli in specialized granulosa cells, we are able to show that some of the mannose-binding sites are associated with ribosomal precursors.
通过甘露糖基化铁蛋白在超微结构水平上检测到蜥蜴颗粒细胞原位细胞核中的甘露糖结合位点。当用甘露糖基化铁蛋白孵育包埋在乙二醇甲基丙烯酸酯中的材料的超薄切片时，在核仁、染色质和核膜外小叶上观察到强烈的标记，但除核孔外，在核周空间未检测到标记。当切片在含有甘露糖基化铁蛋白和一种糖相关新糖蛋白（甘露糖基化血清白蛋白）的溶液中孵育时，这种标记明显受到抑制。这些支持爬行动物细胞中存在核凝集素样成分的超微结构数据与我们之前关于从哺乳动物细胞分离的细胞核中此类成分的发现一致。由于特殊颗粒细胞中核仁颗粒成分的独特组织，我们能够证明一些甘露糖结合位点与核糖体前体相关。
