Tsuda H, Sugihara S, Nishida H, Hara H, Eriguchi N, Ishii K, Sasaki K, Yoshimura S, Tanaka N
Department of Anesthesiology, Kurume University, School of Medicine, Fukuoka-ken.
Jpn J Cancer Res. 1992 May;83(5):527-31. doi: 10.1111/j.1349-7006.1992.tb01960.x.
The inhibitory effects of a combination of Antineoplaston A-10 Injection with a small dose of cis-diamminedichloroplatinum (CDDP) on cell and tumor growth was tested in vitro and in vivo settings. A human hepatocellular carcinoma cell line (KIM-1) was used for the cell growth and transplanted tumor growth studies. In the cell growth study, one-hour exposure of KIM-1 cells to CDDP in the medium at concentrations of 0.5, 1.0, and 2.0 micrograms/ml inhibited cell growth dose-dependently. Continuous exposure of cultured cells to Antineoplaston A-10 Injection at concentrations of 4, 6, and 8 mg/ml also inhibited tumor growth dose-dependently. The combination of 0.5 microgram/ml CDDP and 6 mg/ml A-10 Injection inhibited cell growth more than did each agent individually. Electron microscopic study showed well-maintained organelle structures in Antineoplaston A-10 Injection-treated cells compared to CDDP-treated cells. alpha-Fetoprotein (AFP) production by 10(4) cells in 48 h increased in the A-10 Injection-treated and A-10 Injection+CDDP-treated groups as the concentration of these agents increased. In the tumor growth study, daily administration of Antineoplaston A-10 Injection 75 mg with once a week administration of 20 micrograms of CDDP for 5 weeks inhibited transplanted tumor growth in athymic mice after 33 days of treatment, while administration of 75 mg of A-10 Injection or 20 or 60 micrograms of CDDP alone showed no significant inhibition of tumor growth.
在体外和体内环境中测试了抗癌灵A - 10注射液与小剂量顺二氨二氯铂（CDDP）联合使用对细胞和肿瘤生长的抑制作用。使用人肝癌细胞系（KIM - 1）进行细胞生长和移植瘤生长研究。在细胞生长研究中，将KIM - 1细胞在含有浓度为0.5、1.0和2.0微克/毫升CDDP的培养基中暴露1小时，细胞生长呈剂量依赖性抑制。将培养细胞持续暴露于浓度为4、6和8毫克/毫升的抗癌灵A - 10注射液中，肿瘤生长也呈剂量依赖性抑制。0.5微克/毫升CDDP与6毫克/毫升A - 10注射液联合使用比单独使用每种药物对细胞生长的抑制作用更强。电子显微镜研究显示，与CDDP处理的细胞相比，抗癌灵A - 10注射液处理的细胞细胞器结构保持良好。随着这些药物浓度的增加，在抗癌灵A - 10注射液处理组和抗癌灵A - 10注射液 + CDDP处理组中，48小时内10⁴个细胞的甲胎蛋白（AFP）产量增加。在肿瘤生长研究中，在无胸腺小鼠中，每周一次给予20微克CDDP，持续5周，同时每天给予75毫克抗癌灵A - 10注射液，治疗33天后抑制了移植瘤生长，而单独给予75毫克A - 10注射液或20或60微克CDDP均未显示出对肿瘤生长的显著抑制作用。
