使用聚合酶链反应鉴定布鲁氏菌属。
Identification of Brucella spp. by using the polymerase chain reaction.
作者信息
Herman L, De Ridder H
机构信息
Rijkszuivelstation, Centrum voor Landbouwkundig Onderzoek, Melle, Belgium.
出版信息
Appl Environ Microbiol. 1992 Jun;58(6):2099-101. doi: 10.1128/aem.58.6.2099-2101.1992.
Abstract
The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.
摘要
本文介绍了两种合成寡核苷酸作为探针和引物在聚合酶链反应中的应用，用于布鲁氏菌属的特异性、灵敏且快速的鉴定。基于流产布鲁氏菌和根癌土壤杆菌之间的16S rRNA序列比对选择了特异性寡核苷酸序列。
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