一种用于检测……的新型聚合酶链反应检测方法 （原文最后不完整，翻译可能存在部分不准确，需完整原文才能精确翻译）
A Novel PCR Assay for Detecting and .
作者信息
Alamian Saeed, Esmaelizad Majid, Zahraei Taghi, Etemadi Afshar, Mohammadi Mohsen, Afshar Davoud, Ghaderi Soheila
机构信息
Department of Brucellosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
Department of Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
出版信息
Osong Public Health Res Perspect. 2017 Feb;8(1):65-70. doi: 10.24171/j.phrp.2017.8.1.09. Epub 2017 Feb 28.
OBJECTIVES
Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.
METHODS
All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate from . A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013-2014.
RESULTS
Biochemical tests and bacteriophage typing as the golden standard indicated that all spp. isolates were biovar 1 and biovar 3. The PCR results were the same as the bacteriological method for detecting spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting and .
CONCLUSION
Quick detection of and can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both and are detectable in a single test tube. Furthermore, this method covered 100% of all and biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of and .
目的
布鲁氏菌病是一种主要的人畜共患病，在全球范围内对公共卫生构成重大威胁。用于鉴定布鲁氏菌属物种的经典细菌学检测过程既困难又耗时。本研究旨在开发一种用于检测布鲁氏菌病的新型分子检测方法。
方法
比较了所有可用布鲁氏菌序列中长度为2.1 Mbp的1号染色体的所有完整序列。1号染色体上的一个独特重复序列（URS）位点可区分流产布鲁氏菌和马尔他布鲁氏菌。设计了一组引物，使其位于该独特位点两侧。2013年至2014年，共对136份淋巴结和血液样本采用URS聚合酶链反应（PCR）方法进行评估和分类。
结果
作为金标准的生化试验和噬菌体分型表明，所有布鲁氏菌属分离株均为流产布鲁氏菌生物变种1和马尔他布鲁氏菌生物变种3。PCR结果与检测布鲁氏菌属物种的细菌学方法相同。URS-PCR方法的敏感性和特异性使其适用于检测流产布鲁氏菌和马尔他布鲁氏菌。
结论
快速检测流产布鲁氏菌和马尔他布鲁氏菌可为控制这些细菌提供最有效的策略。该方法相对于其他现有方法的优势在于，可在单个试管中同时检测流产布鲁氏菌和马尔他布鲁氏菌。此外，该方法涵盖了所有流产布鲁氏菌和马尔他布鲁氏菌生物型的100%。这种URS-PCR方法的开发是朝着开发一种用于流产布鲁氏菌和马尔他布鲁氏菌分子鉴定的新型试剂盒迈出的第一步。
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