用于从感染动物的血液和乳汁中检测布鲁氏菌属的一步法聚合酶链反应。
Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.
作者信息
Leal-Klevezas D S, Martínez-Vázquez I O, López-Merino A, Martínez-Soriano J P
机构信息
Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, Mexico.
出版信息
J Clin Microbiol. 1995 Dec;33(12):3087-90. doi: 10.1128/jcm.33.12.3087-3090.1995.
Abstract
A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.
摘要
本文介绍了一种通用的布鲁氏菌DNA提取方法以及聚合酶链反应（PCR），它们是从受感染动物体液中检测布鲁氏菌属的可靠工具。设计了两条与编码外膜蛋白（omp-2）基因区域同源的寡核苷酸，用于从受感染山羊、牛和人类患者的牛奶和/或血液中检测病原体。我们检测方法的灵敏度及其在现场样本中检测病原体的能力，揭示了动物和人类布鲁氏菌病诊断方面的一项有前景的进展。
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