人和大鼠脑肌醇单磷酸酶的cDNA克隆。人重组酶的表达与特性分析。

McAllister G, Whiting P, Hammond E A, Knowles M R, Atack J R, Bailey F J, Maigetter R, Ragan C I

Merck Sharp and Dohme Research Laboratories, Harlow, Essex, U.K.

Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):749-54. doi: 10.1042/bj2840749.

Inositol monophosphatase (EC 3.1.3.25) is a key enzyme in the phosphoinositide cell-signalling system. Its role is to provide inositol required for the resynthesis of phosphatidylinositol and polyphosphoinositides. It is the probable pharmacological target for lithium action in brain. Using probes derived from the bovine inositol monophosphatase cDNA we have isolated cDNA clones encoding the human and rat brain enzymes. The enzyme is highly conserved in all three species (79% identical). The coding region of the human cDNA was inserted into a bacterial expression vector. The expressed recombinant enzyme was purified and its biochemical properties examined. The human enzyme is very similar to the bovine enzyme.

肌醇单磷酸酶（EC 3.1.3.25）是磷酸肌醇细胞信号系统中的关键酶。其作用是提供磷脂酰肌醇和多磷酸肌醇再合成所需的肌醇。它可能是锂在脑中发挥作用的药理学靶点。利用从牛肌醇单磷酸酶cDNA衍生的探针，我们分离出了编码人和大鼠脑内该酶的cDNA克隆。该酶在所有这三个物种中高度保守（同一性为79%）。将人cDNA的编码区插入细菌表达载体。对表达的重组酶进行纯化并检测其生化特性。人酶与牛酶非常相似。