Tee W, Lambert J, Smallwood R, Schembri M, Ross B C, Dwyer B
Clinical Pathology Laboratory, Fairfield Infectious Diseases Hospital, Australia.
J Clin Microbiol. 1992 Jun;30(6):1562-7. doi: 10.1128/jcm.30.6.1562-1567.1992.
Ribotyping is a method used to type strains of bacteria by analyzing the restriction enzyme digestion patterns of the rRNA genes. This method was applied to 126 strains of Helicobacter pylori from 100 unrelated symptomatic patients who had endoscopies done and to 15 strains from 15 infected subjects from seven families. Analysis of the rRNA gene patterns revealed 77 distinct ribotypes from the 100 patients. From 15 of these subjects, isolates were recovered from antral mucosal biopsies at follow-up endoscopy. All follow-up isolates from the same patient, with one exception, yielded identical digest patterns. This patient had strains with two distinct digest patterns obtained from a set of three isolates cultured from biopsy specimens taken at different times. Five patients who had isolates recovered from different sites in the stomach (antrum, gastric body, duodenum, and pyloric channel) showed ribotyping patterns which were identical for each patient yet distinct between patients. In seven family groups studied, identical digest patterns were detected in members of two families, with variability in strains detected among members of the remaining families. This study demonstrates that ribotyping provides a useful, reliable, reproducible, and highly discriminatory typing scheme for the study of H. pylori infection.
核糖体分型是一种通过分析rRNA基因的限制性内切酶消化模式来对细菌菌株进行分型的方法。该方法应用于100例接受内镜检查的有症状的非相关患者的126株幽门螺杆菌，以及来自7个家庭的15名感染受试者的15株菌株。对rRNA基因模式的分析显示，100例患者中有77种不同的核糖体分型。在其中15名受试者中，在随访内镜检查时从胃窦黏膜活检中分离出菌株。除一例例外，同一患者的所有随访分离株产生相同的消化模式。该患者从不同时间采集的活检标本培养的一组三个分离株中获得了具有两种不同消化模式的菌株。从胃的不同部位（胃窦、胃体、十二指肠和幽门管）分离出菌株的5例患者显示，每个患者的核糖体分型模式相同，但患者之间不同。在研究的7个家庭组中，在两个家庭的成员中检测到相同的消化模式，其余家庭的成员中检测到的菌株存在差异。这项研究表明，核糖体分型为幽门螺杆菌感染的研究提供了一种有用、可靠、可重复且具有高度鉴别力的分型方案。
