Owen R J, Bickley J, Lastovica A, Dunn J P, Borman P, Hunton C
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Epidemiol Infect. 1992 Feb;108(1):39-50. doi: 10.1017/s0950268800049487.
Fifty-two strains of Helicobacter pylori were examined by DNA restriction endonuclease digestion, ribosomal (r)RNA gene probe hybridization and biotyping. Most (49) strains originated from gastric (antral) biopsies of patients before or after elective surgery for duodenal ulcers. Chromosomal DNA Hind III ribopatterns showed 9 strain clusters of which the largest contained 12 strains each with 3 common bands (1.50, 3.45, and 4.26 kb) but which were heterogeneous with respect to biotype and total digest pattern. Isolates from post-operative patients with either healed or recurrent ulcers showed ribopattern heterogeneity and exhibited a similar distribution of H. pylori ribopattern types; no single type predominated in any patient group or was more highly associated with recurrent ulcers than with healed ulcers. Multiple isolates from two surgical patients had only minor genomic variations in each set whereas isolates from two brothers had different ribopatterns. We conclude that Hind III ribopatterns in conjunction with total digest patterns might provide the basis for future epidemiological typing studies.
采用DNA限制性内切酶消化、核糖体（r）RNA基因探针杂交和生物分型方法对52株幽门螺杆菌进行了检测。多数（49株）菌株来源于十二指肠溃疡择期手术前后患者的胃（窦）活检标本。染色体DNA Hind III酶切图谱显示有9个菌株簇，其中最大的簇包含12株，各有3条共同条带（1.50、3.45和4.26 kb），但在生物型和总酶切图谱方面存在异质性。来自溃疡已愈合或复发的术后患者的分离株显示酶切图谱存在异质性，且幽门螺杆菌酶切图谱类型的分布相似；在任何患者组中均无单一类型占主导，也没有哪种类型与复发性溃疡的关联比与已愈合溃疡的关联更强。来自两名手术患者的多个分离株在每组中仅有微小的基因组变异，而来自两兄弟的分离株具有不同的酶切图谱。我们得出结论，Hind III酶切图谱结合总酶切图谱可能为未来的流行病学分型研究提供基础。
