一氧化氮合酶的竞争性抑制可防止与外周神经刺激相关的皮质充血。
Competitive inhibition of nitric oxide synthase prevents the cortical hyperemia associated with peripheral nerve stimulation.
作者信息
Northington F J, Matherne G P, Berne R M
机构信息
University of Virginia Health Sciences Center, Department of Physiology, Charlottesville 22908.
出版信息
Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6649-52. doi: 10.1073/pnas.89.14.6649.
With combined microdialysis and hydrogen clearance techniques for simultaneous local delivery of drugs and blood-flow measurement in the rat hindlimb sensory-motor cortex, we examined the role of nitric oxide in cerebral blood-flow regulation during sciatic nerve stimulation. Infusion of 1 mM nitric oxide synthase antagonist, N eta-nitro-L-arginine methyl ester (L-NAME), blocked the cortical blood-flow response to sciatic nerve stimulation (152 +/- 43 ml.min-1.100 g-1 of tissue in controls and 73 +/- 11 ml.min-1.100 g-1 in the presence of L-NAME; P less than 0.05). Addition of 10 mM L-arginine to the dialysate containing L-NAME partially restored the hyperemic response to nerve stimulation (125 ml.min-1.100 g-1). L-NAME also produced a decrease in baseline cerebral blood flow when compared with the control (66 +/- 14 ml.min-1.100 g-1 vs. 93 +/- 25 ml.min-1.100 g-1). We conclude that nitric oxide from activated neurons participates in the local regulation of cortical blood flow in response to sciatic nerve stimulation and also in the maintenance of basal cortical blood flow.
我们采用微透析和氢清除技术相结合的方法，在大鼠后肢感觉运动皮层同时进行药物局部递送和血流量测量，研究了一氧化氮在坐骨神经刺激期间脑血流量调节中的作用。注入1 mM一氧化氮合酶拮抗剂Nω-硝基-L-精氨酸甲酯（L-NAME）可阻断皮层对坐骨神经刺激的血流反应（对照组为152±43 ml·min-1·100 g-1组织，存在L-NAME时为73±11 ml·min-1·100 g-1；P<0.05）。向含有L-NAME的透析液中添加10 mM L-精氨酸可部分恢复对神经刺激的充血反应（125 ml·min-1·100 g-1）。与对照组相比，L-NAME还使基线脑血流量降低（66±14 ml·min-1·100 g-1对93±25 ml·min-1·100 g-1）。我们得出结论，激活神经元产生的一氧化氮参与了对坐骨神经刺激的皮层血流量局部调节，也参与了基础皮层血流量的维持。
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