Makarov E M, Apirion D
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
Biochem Int. 1992 May;26(6):1115-24.
Characterization of the maturation of precursor 10Sa RNA revealed that RNase III processed p10Sa RNA to two intermediate molecules. We showed that the intermediates are not conformers and both are larger than the mature 10Sa RNA. Cell extracts further process the RNase III products to an RNA molecule which has a different conformation than 10Sa RNA but is approximately the same size as 10Sa RNA. An inhibitor of p10Sa RNA processing by RNase III was identified. It is a protein, with a molecular mass of approximately 17 kDa.
前体10Sa RNA成熟过程的表征显示，核糖核酸酶III将p10Sa RNA加工成两个中间分子。我们表明，这些中间体不是构象异构体，且两者都比成熟的10Sa RNA大。细胞提取物进一步将核糖核酸酶III的产物加工成一种RNA分子，其构象与10Sa RNA不同，但大小与10Sa RNA大致相同。鉴定出一种核糖核酸酶III加工p10Sa RNA的抑制剂。它是一种蛋白质，分子量约为17 kDa。
