Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702; email:
Annu Rev Genet. 2013;47:405-31. doi: 10.1146/annurev-genet-110711-155618.
RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes. Recent insight into the mechanism of dsRNA binding and processing, gained from structural studies of RNase III, is reviewed. Structural studies also reveal new cleavage sites in the enzyme that can generate longer 3' overhangs.
RNase III 是大肠杆菌中基因表达的全局调控因子，在核糖体和其他结构 RNA 的成熟中起着重要作用。在这里，我们研究了 RNase III 自身如何响应细胞遇到的生长和其他环境变化进行调节，以及如何通过结合或加工双链 RNA（dsRNA）中间体来控制基因的表达。最近，通过对 RNase III 的结构研究获得了关于 dsRNA 结合和加工机制的新见解，本文对此进行了综述。结构研究还揭示了该酶中的新切割位点，这些位点可以产生更长的 3'突出端。
