Krawczynski K, Beach M J, Bradley D W, Kuo G, di Bisceglie A M, Houghton M, Reyes G R, Kim J P, Choo Q L, Alter M J
Hepatitis Branch, Centers for Disease Control, Atlanta, Georgia.
Gastroenterology. 1992 Aug;103(2):622-9. doi: 10.1016/0016-5085(92)90856-t.
Hepatitis C virus (HCV) antigen was detected immunohistochemically using fluorescein isothiocyanate-labeled immunoglobulin G fractions from chimpanzee and human sera strongly reactive with recombinant hepatitis C virus structural and non-structural proteins. The antigen was localized in the cytoplasm of hepatocytes in all 9 chimpanzees with acute hepatitis C, in 5 of 10 chimpanzees with chronic HCV infection, and in 11 of 12 patients with chronic hepatitis C. The specificity of the hepatocellular HCV and FITC-labeled probes for HCV was ascertained by blocking studies with paired serum samples obtained from 8 infected and uninfected chimpanzees or from 14 patients during the acute and chronic phases of HCV infection. Absorption experiments on FITC-labeled probes with selected host proteins (normal liver homogenate, plasma proteins, red blood cells) did not indicate cross reactivity of the probes with these antigens. Direct immunomorphologic evidence for the HCV specificity of hepatocellular HCV antigen deposits and the FITC-labeled polyclonal anti-HCVAg probe was established in absorption experiments using recombinant HCV nonstructural proteins. The putative HCV NS3 protein was the most prominent component of hepatocellular HCV antigen.
使用来自黑猩猩和人血清的异硫氰酸荧光素标记的免疫球蛋白G组分通过免疫组织化学检测丙型肝炎病毒（HCV）抗原，这些血清与重组丙型肝炎病毒结构和非结构蛋白强烈反应。在所有9只患有急性丙型肝炎的黑猩猩、10只患有慢性HCV感染的黑猩猩中的5只以及12例慢性丙型肝炎患者中的11例中，该抗原定位于肝细胞的细胞质中。通过用从8只感染和未感染的黑猩猩或从14例HCV感染急性和慢性期患者获得的配对血清样本进行封闭研究，确定了肝细胞HCV和FITC标记探针针对HCV的特异性。用选定的宿主蛋白（正常肝匀浆、血浆蛋白、红细胞）对FITC标记探针进行吸收实验，未表明探针与这些抗原存在交叉反应。在使用重组HCV非结构蛋白的吸收实验中，建立了肝细胞HCV抗原沉积物和FITC标记的多克隆抗HCVAg探针针对HCV特异性的直接免疫形态学证据。推测的HCV NS3蛋白是肝细胞HCV抗原的最主要成分。
