在携带亚基因组复制子的Huh-7细胞中鉴定丙型肝炎病毒RNA复制复合体。
Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons.
作者信息
Gosert Rainer, Egger Denise, Lohmann Volker, Bartenschlager Ralf, Blum Hubert E, Bienz Kurt, Moradpour Darius
机构信息
Department of Medicine II, University of Freiburg, Germany.
出版信息
J Virol. 2003 May;77(9):5487-92. doi: 10.1128/jvi.77.9.5487-5492.2003.
Abstract
Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.
摘要
由病毒蛋白、正在复制的RNA和改变的细胞膜组成的膜相关复制复合体的形成是正链RNA病毒的一个特征。在此，我们证明了在含有自主复制的亚基因组丙型肝炎病毒（HCV）RNA的Huh-7细胞中存在一种特定的膜改变，称为膜性网，其含有HCV非结构蛋白以及病毒正链RNA。在放线菌素D存在的情况下用5-溴尿苷5'-三磷酸进行代谢标记表明，膜性网是病毒RNA合成的位点，因此代表了HCV的复制复合体。
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