Walker D H, Liu Q H, Yu X J, Li H, Taylor C, Feng H M
Department of Pathology, University of Texas Medical Branch, Galveston.
Am J Trop Med Hyg. 1992 Jul;47(1):78-86. doi: 10.4269/ajtmh.1992.47.78.
Analysis of seven strains designated as Rickettsia conorii for reactivity with a panel of 12 monoclonal antibodies to surface-protein epitopes of spotted fever group rickettsiae and by Western immunoblotting with standard serotyping sera revealed remarkable antigenic diversity. Rickettsial strains from France, Morocco, Ethiopia, Kenya, South Africa, India, and the USSR differed from one another in reactivity with at least one and as many as five monoclonal antibodies. Simko and Indian strains were similar to one another and differed substantially from other R. conorii strains. All seven strains reacted with three R. conorii-specific monoclonal antibodies. Western immunoblotting demonstrated a major 120-kD protein and a major 135-kD protein in all strains. The principal differences were the presence of a major undenatured 130-kD protein in all strains except Indian and Simko, which had an analogous protein of 124 kD. Immunodominant antigenically related, heat-denatured protein bands of 170 kD (Malish 7 strain), 175 kD (Manuel strain), and 190 kD (Kenya tick typhus, Indian, and Simko strains) were not detected in the M-1 and Moroccan strains. This antigenic diversity is greater than that previously reported for other spotted fever group rickettsial species, suggesting that R. conorrii is an older species than R. rickettsii with a longer period of time for evolutionary divergence.
对七株被指定为康氏立克次体的菌株进行分析，检测其与一组针对斑点热群立克次体表面蛋白表位的12种单克隆抗体的反应性，并通过用标准血清分型血清进行Western免疫印迹分析，结果显示出显著的抗原多样性。来自法国、摩洛哥、埃塞俄比亚、肯尼亚、南非、印度和苏联的立克次体菌株在与至少一种乃至多达五种单克隆抗体的反应性上彼此不同。西姆科菌株和印度菌株彼此相似，与其他康氏立克次体菌株有很大差异。所有七株菌株都与三种康氏立克次体特异性单克隆抗体发生反应。Western免疫印迹分析表明，所有菌株中均有一条主要的120-kD蛋白条带和一条主要的135-kD蛋白条带。主要差异在于，除印度和西姆科菌株（其有一条124 kD的类似蛋白条带）外，所有菌株中均存在一条主要的未变性130-kD蛋白条带。在M-1菌株和摩洛哥菌株中未检测到170 kD（马利什7菌株）、175 kD（曼努埃尔菌株）和190 kD（肯尼亚蜱传斑疹伤寒、印度和西姆科菌株）的免疫显性、抗原相关的热变性蛋白条带。这种抗原多样性大于先前报道的其他斑点热群立克次体物种，这表明康氏立克次体是一个比立氏立克次体更古老的物种，有更长的进化分歧时间。
