逆转录后通过聚合酶链反应对胃癌中c-erbB-2 mRNA表达进行特异性检测。
Specific detection of c-erbB-2 mRNA expression in gastric cancers by the polymerase chain reaction following reverse transcription.
作者信息
Ninomiya I, Endo Y, Yonemura Y, Noguchi M, Fushida S, Nakai M, Takamura H, Harada F, Suzuki T, Miyazaki I
机构信息
Department of Surgery II, School of Medicine, Kanazawa University, Japan.
出版信息
Br J Cancer. 1992 Jul;66(1):84-7. doi: 10.1038/bjc.1992.221.
Abstract
The expression of the c-erbB-2 mRNA in human embryonic lung fibroblasts, a gastric cancer cell line, mature placenta, and 25 gastric cancer tissues was examined by using the polymerase chain reaction following reverse transcription. This technique can be used to examine c-erbB-2 mRNA expression in a small endoscopic biopsy specimen before surgery.
摘要
采用逆转录后的聚合酶链反应检测人胚肺成纤维细胞、一种胃癌细胞系、成熟胎盘以及25例胃癌组织中c-erbB-2 mRNA的表达。该技术可用于在手术前检测小的内镜活检标本中c-erbB-2 mRNA的表达。
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