Fléjou J F, Paraf F, Muzeau F, Fékété F, Hénin D, Jothy S, Potet F
Department of Pathology, Hôpital Beaujon, Clichy, France.
J Clin Pathol. 1994 Jan;47(1):23-6. doi: 10.1136/jcp.47.1.23.
To establish the prevalence of c-erbB-2 protein expression in a surgical series of Barrett's adenocarcinomas; and to correlate this expression with clinicopathological data and prognosis.
Sixty six surgical specimens of Barrett's adenocarcinomas were included in this retrospective study. Blocks of the tumour and of non-dysplastic Barrett's mucosa were stained with a polyclonal antibody specific for the intracytoplasmic domain of the c-erbB-2 protein.
Seven of 66 tumours showed membrane staining for the c-erbB-2 protein. The non-dysplastic Barrett's mucosa was negative in all cases. There was no difference between c-erbB-2 positive and negative tumours with regard to mean age, sex ratio, percentage of alcohol misusers, percentage of smokers, tumour differentiation, depth of invasion, lymph node response, and proliferative activity, assessed by the percentage of tumour cells positive with the MIB-1 antibody directed against the Ki-67 antigen. All c-erb B2 positive tumours were of Lauren's intestinal type compared with negative c-erbB-2 tumours. Patients with c-erbB-2 positive tumours had a significantly poorer prognosis than patients with negative tumours.
The prevalence of Barrett's adenocarcinomas expressing c-erbB-2 found in this study (11%) was similar to that observed in published series of gastric adenocarcinomas. c-erbB-2 protein expression could be an important prognostic indicator in Barrett's adenocarcinoma.
确定手术切除的一系列巴雷特腺癌中c-erbB-2蛋白表达的发生率；并将该表达与临床病理数据及预后相关联。
本回顾性研究纳入了66例巴雷特腺癌手术标本。用针对c-erbB-2蛋白胞质内结构域的多克隆抗体对肿瘤组织块及无发育异常的巴雷特黏膜进行染色。
66例肿瘤中有7例显示c-erbB-2蛋白膜染色。所有病例中无发育异常的巴雷特黏膜均为阴性。通过针对Ki-67抗原的MIB-1抗体检测肿瘤细胞阳性百分比评估，c-erbB-2阳性和阴性肿瘤在平均年龄、性别比例、酗酒者百分比、吸烟者百分比、肿瘤分化程度、浸润深度、淋巴结反应及增殖活性方面均无差异。与c-erbB-2阴性肿瘤相比，所有c-erbB-2阳性肿瘤均为劳伦肠型。c-erbB-2阳性肿瘤患者的预后明显比阴性肿瘤患者差。
本研究中发现的巴雷特腺癌表达c-erbB-2的发生率（11%）与已发表的胃癌系列研究中观察到的相似。c-erbB-2蛋白表达可能是巴雷特腺癌的一个重要预后指标。
