嗜热栖热菌苯丙氨酰-tRNA合成酶识别tRNA（Phe）的核苷酸的确定

Determination of tRNA(Phe) recognition nucleotides for phenylalanyl-tRNA synthetase from Thermus thermophilus.

作者信息

Moor N, Nazarenko I, Ankilova V, Khodyreva S, Lavrik O

机构信息

Institute of Bioorganic Chemistry, Novosibirsk, Russia.

出版信息

Biochimie. 1992 Apr;74(4):353-6. doi: 10.1016/0300-9084(92)90112-r.

DOI:10.1016/0300-9084(92)90112-r

PMID:1379078

Abstract

The tRNA(Phe) recognition nucleotides for phenylalanyl-tRNA synthetase from an extreme thermophile Thermus thermophilus were investigated. Using yeast tRNA(Phe) T7 transcripts with various point mutations it was shown that four recognition points (G34, A35, A36 from anticodon and A73 from acceptor stem) are important for aminoacylation at 37 degrees C. In the case of the 73rd discriminator base A----U, but not A----C, substitution suppresses aminoacylation. Position 20, which proved to be essential for all phenylalanyl-tRNA synthetases so far studied, is not involved in the recognition of tRNA(Phe) by the T thermophilus enzyme.

摘要

对嗜热栖热菌（Thermus thermophilus）苯丙氨酰 - tRNA合成酶识别tRNA（Phe）的核苷酸进行了研究。使用具有各种点突变的酵母tRNA（Phe）T7转录本表明，四个识别位点（来自反密码子的G34、A35、A36和来自受体茎的A73）对于37℃下的氨酰化很重要。在第73位鉴别碱基A替换为U而非A替换为C的情况下，会抑制氨酰化。到目前为止，位置20被证明对所有已研究的苯丙氨酰 - tRNA合成酶都至关重要，但嗜热栖热菌的该酶在识别tRNA（Phe）时不涉及此位置。