嗜热栖热菌苯丙氨酰 - tRNA合成酶对苯丙氨酰 - tRNA的识别。

Recognition of tRNAPhe by phenylalanyl-tRNA synthetase of Thermus thermophilus.

作者信息

Moor N A, Ankilova V N, Lavrik O I

机构信息

Laboratory of Bioorganic Chemistry of Enzymes, Novosibirsk Institute of Bioorganic Chemistry, Russia.

出版信息

Eur J Biochem. 1995 Dec 15;234(3):897-902. doi: 10.1111/j.1432-1033.1995.897_a.x.

DOI:10.1111/j.1432-1033.1995.897_a.x

PMID:8575450

Abstract

The tRNA(Phe) nucleotides required for recognition by phenylalanyl-tRNA synthetase of Thermus thermophilus have been determined using Escherichia coli tRNA(Phe) transcripts with various mutations. The anticodon nucleotides are shown to be the most important recognition elements. The discriminator nucleotide, A73, involved in the recognition set of yeast, E. coli and human phenylalanyl-tRNA synthetases contributes only slightly to tRNA(Phe) recognition by Th. thermophilus phenylalanyl-tRNA synthetase. Nucleotide 20 and some tertiary nucleotides, including the conserved G19.C56 base pair, are proposed to participate in stabilization of the precise tRNA conformation required for efficient aminoacylation. The role of the 3'-CCA terminus, common to all tRNAs, in the specific interaction of tRNA with phenylalanyl-tRNA synthetase is discussed.

摘要

利用具有各种突变的大肠杆菌苯丙氨酰 - tRNA转录本，已确定嗜热栖热菌苯丙氨酰 - tRNA合成酶识别所需的tRNA（Phe）核苷酸。反密码子核苷酸被证明是最重要的识别元件。参与酵母、大肠杆菌和人苯丙氨酰 - tRNA合成酶识别组的鉴别核苷酸A73，对嗜热栖热菌苯丙氨酰 - tRNA合成酶识别tRNA（Phe）的贡献很小。有人提出核苷酸20和一些三级核苷酸，包括保守的G19·C56碱基对，参与稳定有效氨酰化所需的精确tRNA构象。讨论了所有tRNA共有的3'-CCA末端在tRNA与苯丙氨酰 - tRNA合成酶特异性相互作用中的作用。