[3'-CCA序列在大肠杆菌和嗜热栖热菌的tRNA（苯丙氨酸）与同源苯丙氨酰-tRNA合成酶相互作用中的作用]
[The role of the 3'-CCA sequence in the interaction of tRNA(Phe) from E. coli and Thermus thermophilus with homologous phenylalanyl-tRNA-synthetases].
作者信息
Moor N A, Stepanov V G, Repkova M N, Ven'iaminova A G, Vratskikh L V, Iamkovoĭ V I, Motorin Iu A, Lavrik O I
出版信息
Biokhimiia. 1994 Sep;59(9):1299-303.
The 3'-CCA end of tRNA(Phe) from E. coli and Thermus thermophilus was modified by stepwise degradation and ligation of the shortened tRNA with different trinucleotides (pUpUpA, (pA)3, (pC)3, (pU)3). Kinetic parameters for the aminoacylation reaction of modified tRNAs have been determined. The role of the 3'-terminal trinucleotide of tRNA(Phe) in tRNA binding and aminoacylation by phenylalanyl-tRNA synthetases from E. coli and Thermus thermophilus is postulated.
通过对来自大肠杆菌和嗜热栖热菌的tRNA（苯丙氨酸）的3'-CCA末端进行逐步降解，并将缩短的tRNA与不同的三核苷酸（pUpUpA、(pA)3、(pC)3、(pU)3）进行连接来对其进行修饰。已测定修饰后tRNA氨基酰化反应的动力学参数。推测了tRNA（苯丙氨酸）的3'-末端三核苷酸在大肠杆菌和嗜热栖热菌苯丙氨酰-tRNA合成酶结合tRNA及氨基酰化过程中的作用。
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