Bengochea L A, Ouviña G B, Pavese A, Lemberg A
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.
Arch Int Physiol Biochim Biophys. 1992 Mar-Apr;100(2):109-11. doi: 10.3109/13813459209035271.
In the rat, the effect of the bile duct ligation on liver microsomal phospholipid fatty acid composition and on phosphatidylcholine, phosphatidylserine and phosphatidylinositol pattern were studied. After two days of cholestasis, microsomal phospholipid fatty acids showed a decrease in linoleic, stearic and arachidonic acids and an increase in oleic and docosahexaenoic ones, as compared to controls. Phosphatidylcholine showed an increment in oleic and palmitic acid content and a concomitant decrease in arachidonic acid. Phosphatidylserine showed a progressive increase while phosphatidylinositol showed a progressive decrease in all fatty acids. Eight-days post-cholestatic rats showed a marked increase in oleic acid, whereas linoleic, arachidonic, stearic and palmitic acids concentration decreased. Phosphatidylcholine showed a global decrease in its fatty acid content, except for oleic which is increased. Phosphatidylserine showed an increase over the two-days cholestasis fatty acids values. Phosphatidylinositol decreased in most fatty acids except in docosahexaenoic acid that recovered normal values. It was concluded that cholestasis produced significative changes in the fatty acid composition of the major phospholipids constituents of the microsomal membranes.
在大鼠中，研究了胆管结扎对肝微粒体磷脂脂肪酸组成以及对磷脂酰胆碱、磷脂酰丝氨酸和磷脂酰肌醇模式的影响。胆汁淤积两天后，与对照组相比，微粒体磷脂脂肪酸中亚油酸、硬脂酸和花生四烯酸减少，油酸和二十二碳六烯酸增加。磷脂酰胆碱中油酸和棕榈酸含量增加，同时花生四烯酸减少。磷脂酰丝氨酸呈逐渐增加趋势，而磷脂酰肌醇的所有脂肪酸均呈逐渐减少趋势。胆汁淤积八天后的大鼠油酸显著增加，而亚油酸、花生四烯酸、硬脂酸和棕榈酸浓度降低。磷脂酰胆碱除油酸增加外其脂肪酸含量总体下降。磷脂酰丝氨酸比胆汁淤积两天时的脂肪酸值有所增加。磷脂酰肌醇在大多数脂肪酸中减少，但二十二碳六烯酸恢复到正常水平。得出的结论是，胆汁淤积使微粒体膜主要磷脂成分的脂肪酸组成发生了显著变化。
