Fucić A, Garaj-Vrhovac V, Skara M, Dimitrovic B
Institute for Medical Research and Occupational Health, University of Zagreb, Croatia.
Mutat Res. 1992 Aug;282(4):265-71. doi: 10.1016/0165-7992(92)90133-3.
Chromosome aberration assays, sister-chromatid exchange techniques and micronucleus assays are commonly used methods for biomonitoring genetic material damaged by chemical or physical agents. On the other hand, their aneugenic activity, which can lead to hypoploidy and may also be associated with carcinogenesis, has not been thoroughly investigated. In our study we chose the micronucleus assay with a new mathematical approach to separate clastogenic from aneugenic activity of three well-known mutagens (vinyl chloride monomer, X-rays and microwaves) on the genome of human somatic cells. The comparison of frequencies of size distribution of micronuclei in the lymphocytes of humans exposed to each of these three mutagens showed that X-rays and microwaves were preferentially clastogens while vinyl chloride monomer showed aneugenic activity as well. Microwaves possess some mutagenic characteristics typical of chemical mutagens.
染色体畸变分析、姐妹染色单体交换技术和微核试验是生物监测化学或物理因子所致遗传物质损伤的常用方法。另一方面，它们的非整倍体活性，可导致亚倍体，也可能与致癌作用相关，但尚未得到充分研究。在我们的研究中，我们选择微核试验，并采用一种新的数学方法，来区分三种著名诱变剂（氯乙烯单体、X射线和微波）对人类体细胞基因组的致断裂活性和非整倍体活性。对接触这三种诱变剂中每一种的人类淋巴细胞微核大小分布频率进行比较，结果表明，X射线和微波主要是致断裂剂，而氯乙烯单体也表现出非整倍体活性。微波具有一些典型化学诱变剂的诱变特性。
