全眼球的微波刺激化学固定
Microwave-stimulated chemical fixation of whole eyes.
作者信息
Margo C E, Saxe S, Grossniklaus H E
机构信息
Department of Ophthalmology, University of Florida College of Medicine, Gainesville.
出版信息
Ophthalmology. 1992 Jul;99(7):1117-22. doi: 10.1016/s0161-6420(92)31863-9.
BACKGROUND
Microwave-stimulated chemical fixation has been used successfully to rapidly prepare nonocular tissue for processing and paraffin embedding.
METHOD
This same technique was adapted to fix whole eyes using a common domestic microwave oven. Histologic sections were compared with sections from globes that were fixed in a traditional manner.
RESULTS
Histologic sections and histochemical and immunohistochemical staining of eyes treated with microwave-stimulated chemical fixation in 10% neutral-buffered formalin were comparable in quality to eyes fixed in 10% neutral-buffered formalin at room temperature for 48 hours.
CONCLUSION
Because microwave irradiation accelerates the most time-consuming part of preparing whole eyes for light microscopy, the technique enhances laboratory efficiency. The technique is particularly well suited for ensuring adequate fixation of globes when histologic diagnosis is needed in less than 48 hours.
背景
微波刺激化学固定已成功用于快速制备非眼部组织以进行处理和石蜡包埋。
方法
采用相同技术，使用普通家用微波炉固定全眼球。将组织学切片与以传统方式固定的眼球切片进行比较。
结果
在10%中性缓冲福尔马林中经微波刺激化学固定处理的眼球，其组织学切片以及组织化学和免疫组织化学染色质量与在室温下于10%中性缓冲福尔马林中固定48小时的眼球相当。
结论
由于微波照射加速了全眼球用于光学显微镜检查制备过程中最耗时的部分，该技术提高了实验室效率。当需要在不到48小时内进行组织学诊断时，该技术特别适合确保眼球的充分固定。
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