Kahveci Z, Cavuşoğlu I, Sirmali S A
Uludağ University Faculty of Medicine, Department of Histology and Embryology, Bursa, Turkey.
Biotech Histochem. 1997 May;72(3):144-7. doi: 10.3109/10520299709082228.
This study compares microwave fixation of whole fetal specimens with conventional techniques performed at room temperature. All fetuses were obtained from the same pregnant rat; half of them were placed in neutral formalin for 15 min at room temperature, then irradiated for 2.5 min in a domestic microwave oven. The remaining fetuses were placed in neutral formalin at room temperature for 48 hr as a control. Both experimental and control groups were exposed to routine tissue processing for light microscopy and embedded in paraffin wax. Sections 5 microns thick were stained with hematoxylin and eosin. Our results showed that the microwave technique reduced the fixation time while providing thin sections that were equal to or better in quality than those in the control group.
本研究将全胎标本的微波固定与室温下的传统技术进行了比较。所有胎儿均取自同一怀孕大鼠；其中一半在室温下置于中性福尔马林中15分钟，然后在家用微波炉中照射2.5分钟。其余胎儿在室温下置于中性福尔马林中48小时作为对照。实验组和对照组均进行常规组织处理以用于光学显微镜检查，并嵌入石蜡中。5微米厚的切片用苏木精和伊红染色。我们的结果表明，微波技术缩短了固定时间，同时提供了与对照组质量相当或更好的薄切片。
