• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Immune electron microscopic characterization of monoclonal antibodies to Alzheimer neurofibrillary tangles.

作者信息

Wrzolek M A, Merz P A, Kascsak R, Grundke-Iqbal I, Iqbal K, Rubenstein R, Tonna-DeMasi M, Goller N L, Mehta P, Wisniewski H M

机构信息

New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10301.

出版信息

Am J Pathol. 1992 Aug;141(2):343-55.

PMID:1379780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1886595/
Abstract

Characterization of eleven monoclonal antibodies (MAbs), raised to isolated sodium dodecyl sulfate (SDS)-treated Alzheimer's neurofibrillary tangles (ANT), has revealed the presence of at least two different epitopes. MAbs were tested for reactivity to ubiquitin and paired helical filaments (PHF) isolated by three different procedures. The effect of protease and/or alkaline phosphatase pretreatment on the reactivity of the MAbs with isolated PHF was also examined. All MAbs that had reacted strongly in the ELISA with sonicated SDS-treated ANT also immune decorated isolated PHF to varying degrees. Two MAbs exhibited a high reactivity to PHF: 3-39 and 5-25. MAb 3-39 was found to recognize a protease sensitive epitope. In contrast MAb 5-25 was found to consistently decorate isolated PHF in all preparations and exhibited a strong reactivity to ubiquitin, and the epitope in isolated PHF was not protease sensitive. Thus structural PHF after protease treatment and detergent treatment contain an antigenic site that is present in ubiquitin.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/1001dbad4cff/amjpathol00080-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/edf5f6fbc3a1/amjpathol00080-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/439ca83df466/amjpathol00080-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/e93e6bc05933/amjpathol00080-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/b15e6ae81dab/amjpathol00080-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/1001dbad4cff/amjpathol00080-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/edf5f6fbc3a1/amjpathol00080-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/439ca83df466/amjpathol00080-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/e93e6bc05933/amjpathol00080-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/b15e6ae81dab/amjpathol00080-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8647/1886595/1001dbad4cff/amjpathol00080-0085-a.jpg

相似文献

1
Immune electron microscopic characterization of monoclonal antibodies to Alzheimer neurofibrillary tangles.
Am J Pathol. 1992 Aug;141(2):343-55.
2
Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites.独特的阿尔茨海默病双螺旋丝特异性表位涉及特定位点的双重磷酸化。
Biochemistry. 1997 Jul 1;36(26):8114-24. doi: 10.1021/bi970380+.
3
Neurofilament monoclonal antibodies RT97 and 8D8 recognize different modified epitopes in paired helical filament-tau in Alzheimer's disease.神经丝单克隆抗体RT97和8D8识别阿尔茨海默病中配对螺旋丝- tau的不同修饰表位。
J Neurochem. 1993 Apr;60(4):1372-82. doi: 10.1111/j.1471-4159.1993.tb03298.x.
4
Alzheimer's disease: a monoclonal antibody raised against paired helical filaments.
Acta Histochem Suppl. 1991;41:143-52.
5
Alzheimer's disease: immunoreactivity of neurofibrillary tangles with anti-neurofilament and anti-paired helical filament antibodies.阿尔茨海默病：神经原纤维缠结与抗神经丝抗体和抗双螺旋丝抗体的免疫反应性。
Brain Res. 1984 Sep 24;310(2):249-60. doi: 10.1016/0006-8993(84)90148-3.
6
Ubiquitin is a component of paired helical filaments in Alzheimer's disease.泛素是阿尔茨海默病中双螺旋丝的一个组成部分。
Science. 1987 Mar 27;235(4796):1641-4. doi: 10.1126/science.3029875.
7
Alzheimer's paired helical filaments: amyloid precursor protein epitope mapping.
Brain Res Bull. 1994;33(4):387-92. doi: 10.1016/0361-9230(94)90281-x.
8
Microtubule-associated polypeptides tau are altered in Alzheimer paired helical filaments.微管相关多肽tau在阿尔茨海默病的双螺旋丝中发生改变。
Brain Res. 1988 Aug;464(1):43-52. doi: 10.1016/0169-328x(88)90017-4.
9
Tau immunoreactivity and SDS solubility of two populations of paired helical filaments that differ in morphology.形态不同的两种成对螺旋丝群体的tau免疫反应性和SDS溶解性。
Brain Res. 1994 Jun 27;649(1-2):185-96. doi: 10.1016/0006-8993(94)91063-4.
10
Rabbit IgG cross-reacts with Alzheimer neurofibrillary tangles.兔免疫球蛋白G与阿尔茨海默病神经原纤维缠结发生交叉反应。
Acta Neuropathol. 1994;87(5):443-9. doi: 10.1007/BF00294170.

引用本文的文献

1
Production of Monoclonal Antibodies to Pathologic β-sheet Oligomeric Conformers in Neurodegenerative Diseases.生产针对神经退行性疾病中病理性 β-折叠寡聚构象的单克隆抗体。
Sci Rep. 2017 Aug 29;7(1):9881. doi: 10.1038/s41598-017-10393-z.

本文引用的文献

1
Immunostaining of neurofibrillary tangles in Alzheimer's senile dementia with a neurofilament antiserum.用神经丝抗血清对阿尔茨海默病性老年痴呆中的神经原纤维缠结进行免疫染色。
J Neurosci. 1982 Jan;2(1):113-9. doi: 10.1523/JNEUROSCI.02-01-00113.1982.
2
Alzheimer neurofibrillary tangles: monoclonal antibodies to inherent antigen(s).阿尔茨海默病神经原纤维缠结：针对内在抗原的单克隆抗体。
Acta Neuropathol. 1984;62(4):268-75. doi: 10.1007/BF00687608.
3
Antibodies to neurofibrillary tangles of Alzheimer's disease raised from human and animal neurofilament fractions.
从人和动物神经丝组分中产生的针对阿尔茨海默病神经原纤维缠结的抗体。
Lab Invest. 1983 Oct;49(4):430-5.
4
Ultrastructure of paired helical filaments of Alzheimer's neurofibrillary tangle.阿尔茨海默病神经原纤维缠结的双螺旋丝超微结构
J Neuropathol Exp Neurol. 1984 Nov;43(6):643-56. doi: 10.1097/00005072-198411000-00008.
5
Alzheimer paired helical filaments: bulk isolation, solubility, and protein composition.阿尔茨海默病配对螺旋丝：大量分离、溶解性及蛋白质组成
Acta Neuropathol. 1984;62(3):167-77. doi: 10.1007/BF00691849.
6
Alzheimer paired helical filaments: immunochemical identification of polypeptides.阿尔茨海默病配对螺旋丝：多肽的免疫化学鉴定
Acta Neuropathol. 1984;62(4):259-67. doi: 10.1007/BF00687607.
7
Antibodies to paired helical filaments in Alzheimer's disease do not recognize normal brain proteins.阿尔茨海默病中针对双螺旋丝的抗体不能识别正常脑蛋白。
Nature. 1983;304(5928):727-30. doi: 10.1038/304727a0.
8
Comparative studies on nucleoside diphosphatase of rat ascites hepatoma and rat liver: activity level, purification and properties.
Int J Biochem. 1982;14(12):1075-81. doi: 10.1016/0020-711x(82)90164-1.
9
Pick's disease: an immunocytochemical study of neuronal changes. Monoclonal antibodies show that Pick bodies share antigenic determinants with neurofibrillary tangles and neurofilaments.皮克病：神经元变化的免疫细胞化学研究。单克隆抗体显示皮克小体与神经原纤维缠结和神经丝具有共同的抗原决定簇。
Acta Neuropathol. 1983;60(3-4):175-82. doi: 10.1007/BF00691864.
10
Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants.单克隆抗体表明神经原纤维缠结和神经丝具有共同的抗原决定簇。
Nature. 1982 Jul 1;298(5869):84-6. doi: 10.1038/298084a0.